Cambio - Excellence in Molecular Biology

Enzymes for Molecular Biology

Enzymes for Molecular Biology: Enzymes for Molecular Biology

RNase-Free DNase I

RNase-Free DNase I (bovine pancreas) is an endonuclease useful in removing DNA that might interfere with the characterisation, manipulation, or use of RNA, or for any application requiring highly purified DNase I

BioSearch Tech (Lucigen/Epicentre)

Catalogue No.DescriptionPack SizePriceQty
D9905KRNase-Free DNase I - supplied at a concentration of 1U/µl.5,000MBU £124.00£124.00Offer until : 31-Dec-2020Contact Cambio for special pricing on all orders over £2000 View Offer Quantity Add to Order
D9910KRNase-Free DNase I - supplied at a concentration of 1U/µl.10,000MBU (2x5,000 MBU) £196.00£196.00Offer until : 31-Dec-2020Contact Cambio for special pricing on all orders over £2000 View Offer Quantity Add to Order

RNase-Free DNase I

RNase-Free DNase I (bovine pancreas) is an endonuclease useful in removing DNA that might interfere with the characterisation, manipulation, or use of RNA, or for any application requiring highly purified DNase I

BioSearch Tech (Lucigen/Epicentre)

RNase-Free DNase I (bovine pancreas) is an endonuclease useful in removing DNA that might interfere with the characterisation, manipulation, or use of RNA, or for any application requiring highly purified DNase I. It efficiently hydrolyses double- and single-stranded DNA to a mixture of short oligo- and mononucleotides. 

RNase free DNASE_1
Figure 1. DNA removal from in vitro transcription reactions using RNase-Free DNase I. A linearized DNA template was transcribed using T7 RNA polymerase according to standard in vitro transcription conditions. Lane 1, kb ladder; Lane 2, DNA control; Lane 3, transcription mixture; Lane 4, transcription mixture treated with 1 MBU of RNase-Free DNase I for 15 minutes at 37°C.

Unit Definition

Using the standard 50µl assay volume, one Molecular Biology Unit (MBU) digests 1µg of pUC79 DNA to oligodeoxynucleotides in 10 minutes at 37°C in 33 mM Tris-acetate, pH 7.8, 66mM potassium acetate, 10mM magnesium acetate, and 0.5mM DTT 2mM CaCl2. 

Storage Buffer

50% glycerol containing 10mM Tris-HCl, pH 7.5, 10mM CaCl2, and 10mM MgCl2. 

Quality Control

No degradation of 1µg of a synthetic RNA transcript is detected by agarose gel electrophoresis following incubation with 10U of RNase-Free DNase I at 37°C for 1 hour.




If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

RNase-Free DNase I

RNase-Free DNase I (bovine pancreas) is an endonuclease useful in removing DNA that might interfere with the characterisation, manipulation, or use of RNA, or for any application requiring highly purified DNase I

BioSearch Tech (Lucigen/Epicentre)

Protocols for: RNase-Free DNase I

RNase-Free DNase I Protocol

(catalogue number D9902K / D9905K / D9910K) 

Please note: all protocols off site are the responsibility of the products supplier

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

RNase-Free DNase I

RNase-Free DNase I (bovine pancreas) is an endonuclease useful in removing DNA that might interfere with the characterisation, manipulation, or use of RNA, or for any application requiring highly purified DNase I

BioSearch Tech (Lucigen/Epicentre)

References

  1. Kienzle, N. et al. (1996) BioTechniques 20, 612.
  2. Sambrook, J. et al. (1989) in: Molecular Cloning: A Laboratory Manual (2nd ed.) Cold Spring Harbor Laboratory Press, New York.
  3. Galas, D.J. and Schmitz, A. (1978) Nucleic Acids Res. 5, 3157.

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

RNase-Free DNase I

RNase-Free DNase I (bovine pancreas) is an endonuclease useful in removing DNA that might interfere with the characterisation, manipulation, or use of RNA, or for any application requiring highly purified DNase I

BioSearch Tech (Lucigen/Epicentre)

Epicentre® Forum Get your Epicentre® Forum - hot off the press!
Click here for direct link to Forum listings

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

RNase-Free DNase I

RNase-Free DNase I (bovine pancreas) is an endonuclease useful in removing DNA that might interfere with the characterisation, manipulation, or use of RNA, or for any application requiring highly purified DNase I

BioSearch Tech (Lucigen/Epicentre)

Applications

  • Elimination of template DNA following in vitro synthesis of RNA with T7, SP6, or T3 RNA polymerase.
  • Labeling of DNA by nick translation, in combination with Klenow or other DNA polymerases.
  • Treatment of RNA prior to RT-PCR.1
  • Characterization of DNA-protein interactions by DNase I footprinting.2,3

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200