Cambio - Excellence in Molecular Biology

Oligo Synthesis

Oligo Synthesis : CEPs

Prices quoted are for single packs only. For multiples of the same product please request a quote. Some of Glen's products are hazardous and may be subject to additional shipping charges. Full product information is available on Glen Research's website.

Azobenzene Phosphoramidite

Azobenzene Phosphoramidite

Glen Research

Catalogue No.DescriptionPack SizePriceQty
10-5800-02Azobenzene Phosphoramidite 0.25g £440.00 Quantity Add to Order
10-5800-90Azobenzene Phosphoramidite 100µmoles £160.00 Quantity Add to Order
10-5800-95Azobenzene Phosphoramidite 50µmoles £84.00 Quantity Add to Order

Azobenzene Phosphoramidite

Azobenzene Phosphoramidite

Glen Research

Azobenzene Phosphoramidite

Structure

Catalog Number: 10-5800-xx

Description: Azobenzene Phosphoramidite

3-O-(Dimethoxytrityl)-2-N-(4-carboxyazobenzene)-D-threonin-
1-yl-O-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
Formula: C47H54N5O6P M.W.: 815.94 F.W.: 375.32

Diluent: Anhydrous Acetonitrile
Coupling: 10 minutes
Deprotection: As required by nucleobases. Compatible with Ammonium Hydroxide for 17 hours at room temperature, Ammonium hydroxide/40% methylamine 1:1 (AMA) for 10 minutes at 65 °C or UltraMild deprotection conditions.
Storage: Freezer storage, -10 to -30°C, dry
Stability in Solution: 1-2 days

Labelling for Photo-Regulation of Oligonucleotides

Photo-control, the use of ultraviolet or visible light to control a reaction, has a number of advantages over other external stimuli:

  • Light does not introduce contaminants into the reaction system,
  • Excitation wavelength can be controlled through the design of the photo-responsive molecule
  • It is now straightforward to control irradiation time and/or local excitation.

When a photo-responsive molecule is directly attached to DNA as a receptor, photo-regulation of the bioprocess regulated by that DNA molecule could, in principle, be achieved. Such photo-responsive DNA could also be used as a switch in a DNA-based nano-machine. Professor Hiroyuki Asanuma and his group at the department of Molecular Design and Engineering of the Graduate School of Engineering of the Nagoya University (Japan) have developed an efficient method to achieve this goal. They have attached azobenzene to DNA and made it photo-responsive1,2. Azobenzene is a typical photo-responsive molecule that isomerizes from its planar trans-form to the non-planar cis-form after UV-light irradiation with a wavelength between 300 nm and 400 nm (lmax is around 330 nm). Interestingly, the system reverts from the cis-form to the trans-form after further irradiation with visible light (wavelength over 400 nm). This process is completely reversible, and the azobenzene group does not decompose or induce undesirable side reactions even on repeated trans-cis isomerization. By introducing azobenzenes into DNA through D-threoninol as a linker, Asanuma and co-workers succeeded in achieving photo-regulation of:

  • Formation and dissociation of a DNA duplex3,4 and
  • Transcription by T7-RNA polymerase reaction5,6,7.

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

Azobenzene Phosphoramidite

Azobenzene Phosphoramidite

Glen Research

MSDS

Glen Report 21.2: New Product - Azobenzene phosphoramidite for the introduction of photo-regulated functions in DNA

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

Azobenzene Phosphoramidite

Azobenzene Phosphoramidite

Glen Research

DILUTION/COUPLING DATA

The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.

ABI 392/394
Cat.No. Pack
Size
Grams/
Pack
0.1M Dil.
(mL)
LV40 LV200 40nm 0.2µm 1µm 10µm
Approximate Number of Additions
10-5800-95 50µmoles .041grams .5 3.33 2 1.25 .91 .67 .17
10-5800-90 100µmoles .082grams 1 20 12 7.5 5.45 4 1
10-5800-02 0.25grams .25grams 3.06 88.67 53.2 33.25 24.18 17.73 4.43
Expedite
Cat.No. Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity 50nm 0.2µm 1µm 15µm
Approximate Number of Additions
10-5800-95 50µmoles .041grams .75 .07 8.6 5.38 3.91 .54
10-5800-90 100µmoles .082grams 1.5 .07 23.6 14.75 10.73 1.48
10-5800-02 0.25grams .25grams 4.57 .07 85 53.13 38.64 5.31
Beckman
Cat.No. Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity 30nm 200nm 1000nm

Approximate Number of Additions
10-5800-95 50µmoles .041grams .75 .07 10.2 6.38 4.64

10-5800-90 100µmoles .082grams 1.5 .07 25.2 15.75 11.45

10-5800-02 0.25grams .25grams 4.57 .07 86.6 54.13 39.36

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200