Cambio - Excellence in Molecular Biology

Transfection - Biontex

Transfection - Biontex: General transfection reagents

Broad range transfection reagents for a variety of mammalian and insect cells

Metafectene® SI plus

Metafectene® SI+ for siRNA & miRNA transfection reagent for mammalian cells

Biontex

Catalogue No.DescriptionPack SizePriceQty
SI010-2.0SI buffer (10x)2 ml £45.00£40.50Offer until : 31-Mar-2020Offer Code : BIO10 View Offer Quantity Add to Order
T100-1.0METAFECTENE® SI plus Transfection Reagent for Mammalian Cells with siRNA and miRNA1 ml £219.00£197.10Offer until : 31-Mar-2020Offer Code : BIO10 View Offer Quantity Add to Order
T100-2.0METAFECTENE® SI plus Transfection Reagent for Mammalian Cells with siRNA and miRNA2x 1.0ml £420.00£378.00Offer until : 31-Mar-2020Offer Code : BIO10 View Offer Quantity Add to Order
T100-5.0METAFECTENE® SI plus Transfection Reagent for Mammalian Cells with siRNA and miRNA5x 1.0ml £875.00£787.50Offer until : 31-Mar-2020Offer Code : BIO10 View Offer Quantity Add to Order

Metafectene® SI plus

Metafectene® SI+ for siRNA & miRNA transfection reagent for mammalian cells

Biontex

  • Suitable for siRNA and miRNA transfection
  • Suitable for adherend and suspension cells
  • Free from serum inhibition
  • Optional "Fast Forward" protocol
  • Specific optimization of lipid composition for gene silencing
  • Predefined RNA/lipid ratio results with less optimization effort
  • Efficient knockdown with low reagent and siRNA volumes
  • Rapid protocol: Two interdependent assays within one week

Metafectene® SI? consists of a lipid blend designed and optimized especially for siRNA and miRNA transfection. Unlike for conventional DNA transfection, transport of the nucleic acid into the cell nucleus is not necessary for RNA transfection. The development of Metafectene® SI? was thus primarily aimed at maximizing the endocytosis volume of the lipoplex plus ensuring rapid and complete release of RNA in the cytosol.

High knockdown rates with low RNA and reagent amounts combined with low toxicity make Metafectene® SI? an ideal tool to be used for all cell lines and primary cells.

Screening processes benefit both from this timesaving and from the fact that the lipoplex formation, and thus all pipetting steps, take place in the well of a multiwell culture plate, eliminating the need for rinsing or transfer. siRNA or miRNA transfection can thus be performed during screening with the maximum efficiency and ease.

Comparative Study

Comparison of knock-down of luciferase expression of cells stably expressing luciferase following transfection of anti-Luc-siRNA with METAFECTENE® SI? or a commercial transfection reagent (L2k) under optimized conditions:

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

Metafectene® SI plus

Metafectene® SI+ for siRNA & miRNA transfection reagent for mammalian cells

Biontex

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

Metafectene® SI plus

Metafectene® SI+ for siRNA & miRNA transfection reagent for mammalian cells

Biontex

Application Transfection of eukariotic cells with siRNA or miRNA
Formulation Cationic lipids with colipids in water
Content Lipid formulation / sufficient quantity of SI buffer
Assays Approx. 1000 (96-well) or approx. 250 (24-well) per 1 ml reagent
Shipping At room temperature
Storage ≤ –15°C
Shelf Life 1 year (after date of delivery)

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200