Cambio - Excellence in Molecular Biology

Transfection - Biontex

Transfection - Biontex: General transfection reagents

Broad range transfection reagents for a variety of mammalian and insect cells

Metafectene® FluoR

For visualization of lipofection with DNA

Biontex

Catalogue No.DescriptionPack SizePriceQty
T050-0.5Metafectene® FluoR0.5ml £115.00 Quantity Add to Order
T050-1.0Metafectene® FluoR1.0ml £190.00 Quantity Add to Order

Metafectene® FluoR

For visualization of lipofection with DNA

Biontex

Metafectene® FluoR transfection reagent presents an outstanding method for visualizing the path of the carrier system through the eukaryotic cell and gaining new information from the transfection process, such as findings on the localization of specific components.

Metafectene® FluoR is based on second-generation transfection reagents, of which METAFECTENE® PRO is the best-proven example. The forward protocol used (24-hour incubation of seeded cells prior to transfection) ensures that cells reach the optimum condition for visualization.

The specific lipid composition of Metafectene® FluoR contains a covalent rhodamine B label which is designed not to impair the transfection process.

The two images below show cells transfected using Metafectene® FluoR and pCMV-eGFP. They clearly show the endocytotic uptake of red fluorescent lipoplexes in the cells and their perinuclear accretion. In addition, the superimposed shots of the fluorescence channels enable you to view the incipient expression of GFP by moving the cursor over the desired image.

Metafectene FluoR  Metafectene FluoR 2

 

Structure of Rhodamine BFluorescence spektrum of METAFECTENE® FluoR

 

Structure of rhodamine B label        

Fluorescence spectrum of METAFECTENE® FluoR

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

Metafectene® FluoR

For visualization of lipofection with DNA

Biontex

Protocol can be found here

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

Metafectene® FluoR

For visualization of lipofection with DNA

Biontex

Highlights

  • Visualization of the full transfection process
  • Protocol adapted for cell visualization
    • Low toxicity for optimum cell morphology
    • Cell density optimized for microscope evaluation

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

Metafectene® FluoR

For visualization of lipofection with DNA

Biontex

Product Specifications 
Application Transfection of eukaryotic cells with DNA
Formulation Cationic lipids and colipids in water, covalently bonded rhodamine B
Content Lipid formulation
Fluorescence Exmax = 557 nm; Emmax = 571 nm
Assays Up to 2500 (24-well) or up to 625 (6-well) per 1 ml of reagent
Shipping At room temperature
Storage ≤ –15°C
Shelf life 1 year (after date of delivery)

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200