| Pyrrolo-C-TOM-CE Phosphoramidite |
Catalog Number: 10-3017-xx
Description: Pyrrolo-C-TOM-CE Phosphoramidite
5'-Dimethoxytrityl-[6-methyl-pyrrolo-[2,3-d]-pyrimidine-2(3H)-one]-
2'-O-triisopropylsilyloxymethyl-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite |
| Formula: C52H72N5O9PSi |
M.W.: 970.23 |
F.W.: 343.27 |
Diluent: Anhydrous Acetonitrile |
| Coupling: 6 minute coupling time recommended |
| Deprotection: Ethanolic Ammonium Hydroxide (1:3) at room temperature for 24 hours or as required by the nucleobases. Remove silyl protecting group according to standard RNA procedures. Technical Bulletin |
| Storage: Freezer storage, -10 to -30°C, dry |
| Stability in Solution: 2-3 days |
| Please Note: This product is covered by US Patent No.: 7,144,995. Patented under European patent number 1483280. |
MINOR RNA PHOSPHORAMIDITES (TOM PROTECTED)
Glen Research offers minor RNA phosphoramidites with either TOM or TBDMS protecting groups. 4-Thio-U, 5-Methyl-Cytidine, 2-Aminopurine riboside and 2-Amino-Adenosine are useful for analyzing RNA structure and activity relationships, for example, in ribozyme studies.
Pyrrolo-C is a fluorescent nucleoside whose fluorescence is sensitive to its environment and is ideal for probing RNA structure. It base-pairs as a normal C nucleotide. It is highly fluorescent and its excitation and emission are well to the red of most fluorescent nucleotide analogs, which eliminates or reduces background fluorescence from proteins. Pyrrolo-CTP has potential uses in biological assay development.
rSpacer is used to introduce an abasic site to an RNA sequence.
The protecting scheme for 2,6-Diaminopurine has been changed and the original product (10-3084) has been replaced with the optimized product (10-3085) below.
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