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In Vitro Toxicology

In Vitro Toxicology: Mutagenicity kits

The Ames II / Ames MPF Microfluctuation Assays are all modifications of the traditional Ames plate incorporation assay.

The Ames II assay uses the S. typhimurium strains TA98 and TAMix (a mixture of 6 strains, each detecting a specific base-pair substitution), whereas the Ames MPF® kits are available with the strains TA98, TA100, TA1535, TA1537, E.coli WP2 uvrA or E.coli WP2 pKM101.

 

Benefits of Xenometrix Ames Mutagenicity Assays over traditional Ames Procedures

  • Easy, compact and high throughput liquid format with 384 well plates.

  • Simple detection method with easy "readout" (as compared to the traditional assay)

  • Kits include all five types strains cited in OECD 471 (CoA supplied), quality controlled according to the same guideline 

  • Much less  test compound required.  (10 mg per strain if tested in triplicates, +/- S9, 6 semi-logarithmic compound dilutions)

  • Ames MPF is the preferred system for genotoxic impurities due to its low consumption of compound

  • Significantly less consumption of S9

  • Less environmental pollution due to significantly less "contaminated" waste

  • Less user intervention

  • Download presentation 

Ames II Test Kits

Rapid screening: The Ames II assay comprises the frameshift strain TA98, and TAMix, consisting of 6 histidine auxotrophic Salmonella tester strains, TA7001-TA7006.

Xenometrix AG

Description

Rapid screening:

The Ames II assay comprises the frameshift strain TA98, and TAMix, consisting of 6 histidine auxotrophic Salmonella tester strains, TA7001-TA7006. Each of these mutants can be reverted only by a specific transition or transversion, so that all possible base-pair changes can be detected by TAMix.

TA98 and TAMix may be used for mutagenicity screening because they indicate both frameshift and base-pair mutations.

The TA700x tester strains are not included among the recommended tester strains in the current, formal test guidelines. The Xenometrix Ames MPF 98/100 detects a similar mutational spectrum but contains the traditional Ames tester strains TA98 and TA100.

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