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Aptamers

Aptamers: Catalogue Aptamers

Important information

Do you know what modifications you will need for your aptamers?  see "modifications" section for details

BoNT-B

Botulinum Neurotoxin Type B Aptamer ATW0075

Base Pair Bio

Catalogue No.DescriptionPack SizePriceQty
ATW0075-100BoNT-B 10 AEU (100ug) POA Quantity Add to Order
ATW0075-25BoNT-B 2.5 AEU (25ug) POA Quantity Add to Order

Description

Target:   Botulinum toxin (BoNT-B)

Target Species:   bacterial

Target Type:   protein

Affinity Constant (Kd):   3.2 nM

Default 3' Linker (Spacer) is 10T. Please submit a custom quote request for other options.

Datasheet

Identifiers: BoNT-B aptamer #983

  • Number of DNA nucleotides: 32 bases
  • Molecular weight: 10,412.1 g/mol
  • Target for selection: Botulinum Neurotoxin Type B, Metabiologics Inc.

Aptamer was selected from a randomized 32-mer library against BoNT-B protein. Proprietary methods were then used to select the aptamer. Aptamer folding instruction before use: Once the aptamer is in its working concentration, it needs to be heated to 85-90 ºC for 2 minutes, and then cooled to room temperature before use.

Validation data with BoNT-B by MST (Microscale Thermophoresis) method:

Buffer used for validation: 1X PBS, pH = 7.4, 1 mM MgCl2

Average Kd = 3.2 ± 0.7nM

Please choose your modification and add as an extra catalogue number:
ATWUNMOD - Unmodified
ATWAMINE - Amine modification
ATWBIOTIN - Biotin modification
ATWTHIOL - Thiol modification (extra charge)
ATWFLUOR - Fluor (all) modification (extra charge)

Custom aptamers can be developed; contact us for details

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200

Protocols

Datasheet

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200

Notes

Please note that aptamers have much lower molecular weights than antibodies. Thus, there are typically five to fifteen times as many moles or molecules of aptamers present in a solution containing an equal mass. For example, 100 micrograms of an aptamer is equivalent to 0.5 to 1.5 mg of antibody, depending on the particular aptamer.

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200

Related products

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200