Cambio - Excellence in Molecular Biology

In vitro Transcription

In vitro Transcription: In Vitro Transcription

T7-FlashScribe™ Transcription Kit

T7-FlashScribe™ Transcription Kit is for the production of in vitro transcribed RNA with T7 RNA polymerase and canonical (GAUC) NTPs. A reaction produces 180 µg RNA from 1 µg DNA in 30 minutes.

CellScript

Catalogue No.DescriptionPack SizePriceQty
C-ASF3507T7-FlashScribe™ Transcription Kit50 rxns £212.00 Quantity Add to Order

T7-FlashScribe™ Transcription Kit

T7-FlashScribe™ Transcription Kit is for the production of in vitro transcribed RNA with T7 RNA polymerase and canonical (GAUC) NTPs. A reaction produces 180 µg RNA from 1 µg DNA in 30 minutes.

CellScript

The T7-FlashScribe™ Transcription Kit is specially formulated to enable users to obtain the maximum
possible yields of RNA from an in vitro transcription (IVT) reaction in just 30 minutes. The standard
30 minute, 20 μl reaction will yield up to 180 μg of RNA from 1 μg of the control template. These yields
are made possible by the high-performance properties of the T7-FlashScribe enzyme.


The T7-FlashScribe Transcription Kit produces exceptionally high yields of either long or short transcripts.
The standard reaction can be scaled up to produce milligram amounts of RNA.


T7-FlashScribe IVT RNA can be processed into mRNA (5'-end capped and 3'-end poly(A) tailed) through
the use of CELLSCRIPT's ScriptCap™ m7G Capping System, ScriptCap 2'-O-Methyltransferase Kit and
A-Plus™ Poly(A) Polymerase Tailing Kit (available separately).

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

T7-FlashScribe™ Transcription Kit

T7-FlashScribe™ Transcription Kit is for the production of in vitro transcribed RNA with T7 RNA polymerase and canonical (GAUC) NTPs. A reaction produces 180 µg RNA from 1 µg DNA in 30 minutes.

CellScript

Link to supplier's pdf https://www.cellscript.com/products/018pl0617CS.pdf

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

T7-FlashScribe™ Transcription Kit

T7-FlashScribe™ Transcription Kit is for the production of in vitro transcribed RNA with T7 RNA polymerase and canonical (GAUC) NTPs. A reaction produces 180 µg RNA from 1 µg DNA in 30 minutes.

CellScript

1.Karikó, K. et al., (2008) Molecular Therapy 16, 1833.

2.Anderson, B.R. et al., (2010) Nucl. Acids Res. 17, 5884.

3.Karikó, K. et al., (2005) Immunity 23, 165.

4.Karikó, K. and Weissman, D. (2007) Curr. Opin. Drug Discov. Devel. 10, 523.

5.Sambrook, J. et al., (1989) Molecular Cloning: A Laboratory Manual (2nd ed.), New York, Cold Spring Harbor Laboratory Press.

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

T7-FlashScribe™ Transcription Kit

T7-FlashScribe™ Transcription Kit is for the production of in vitro transcribed RNA with T7 RNA polymerase and canonical (GAUC) NTPs. A reaction produces 180 µg RNA from 1 µg DNA in 30 minutes.

CellScript

Materials Supplied

  • T7-FlashScribe™ Transcription Kit Contents (50 reactions)
  • Kit Component Reagent Volume
  • T7-FlashScribe™ Enzyme Solution 100 μl
  • 10X T7-FlashScribe™ Transcription Buffer 100 μl
  • 100 mM GTP 90 μl
  • 100 mM ATP 90 μl
  • 100 mM UTP 90 μl
  • 100 mM CTP 90 μl
  • 100 mM Dithiothreitol (DTT) 100 μl
  • RNase-Free DNase I, 1 U/μl 50 μl
  • ScriptGuard™ RNase Inhibitor, 40 U/μl 25 μl
  • T7 Control Template DNA, 0.5 μg/μl 20 μl
  • RNase-Free Water 1.4 ml

Store at –20°C in a freezer without a defrost cycle. Do not store at –70°C.

 

Materials Required, but not Supplied

 

• A DNA template for transcription of your RNA of interest
• Materials or kits for purification of the RNA product (For suggestions, see Section C "Purification of the Transcription Product")
• RNase-free TE Buffer (10 mM Tris-HCl, pH 7.5, 1 mM EDTA)

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200