Description
ExprepTM Plasmid SV DNA Purification kit provides a rapid and convenient method for the small and medium scale preparations of plasmid DNA from bacterial cells and it is used to isolate and purify any plasmids from any E. coli strains. ExprepTM Plasmid SV eliminates the need of organic solvent extraction and alcohol precipitation, allowing rapid and convenient preparation from many samples simultaneously. ExprepTM Plasmid SV kit can yield up to 30 ug (mini) of highly purified plasmid DNA and it can be applicable directly for PCR, cloning, automated sequencing, synthesis of labeled hybridization probes and other enzymatic reactions without further manipulation. ExprepTM Plasmid SV Midi kit procedure requires a centrifuge which has a swinging-out bucket running at 4,000 ~ 5,000 xg.
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Features and Benefits
¦ Spin or vacuum column format
¦ Stable and consistent result
¦ Instant use : No need of additional materials
¦ Fast and simple procedure : complete in 25 minutes
¦ High purity : A260/280 = 1.8 ~ 2.0
¦ Compatible with endA strains
¦ No use of organic solvents
¦ Ready for use in enzymatic manipulation and automated sequencing
| Features |
Plasmid SV mini |
Plasmid SV Midi |
|---|
| Format |
Column type Q (mini) with collection tube | Column type Q (Midi) with collection tube |
| Recommended sample volume | 5 ml LB | 50 ml LB |
| Maximum sample volume | Up to 10 ml LB | Up to 100 ml LB |
| Lysate clearing | Centrifugation | EzClear™ Filter |
| Preparation time | under 23 minutes | under 50 minutes |
| Maximum loading volume | 800 μl
| 15 ml |
| Binding capacity | 30 μg | 300 μg |
| Recovery rate | 85~95% | 85~95% |
| Maximum Elution volume | 40 μl | 400 μl |
Technical Data
Stable and Reproducible Result
DNA Automated Squencing Analysis
Compatibility Test with Restriction Enzymes
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