Cambio - Excellence in Molecular Biology

Lab Reagents

Lab Reagents: Smart Buffers and Reagents

Biological buffers for the life science laboratory need to meet a wide array of criteria. These include good stability, lack of toxicity, precision in pH, pKa between 6.0 and 8.0 (the region in which most biological reactions occur), minimal salt effects due to the ionic composition of the solution, free of enzymatic and hydrolytic activity and minimal participation in biological reactions. Buffers for molecular biology applications, e.g. Tris-Borate-EDTA and Tris-Acetate-EDTA, need to be free from DNAse and RNAse activity. Medicago's SmartBuffers meet all these criteria. They are manufactured using highly purified, analytical grade chemicals and are extensively quality tested. Manufacturing is done in a controlled clean environment according to GMP procedures. A high level of automation essentially avoids operator intervention and contact with the products. SmartBuffers are supplied as exactly pre-weighed tablets packed in containers or blister packs or as exactly pre-weighed powder mixes packed in sealed aluminium foil pouches. •Pre-mixed powder buffers or tablets with pre-set pH•Analytical grade reagents •Eliminates calculation, formulation and weighing errors •Dissolve-and-go for greater convenience •Stable at room temperature for at least 3 years •Guaranteed reproducibility

Phosphate Buffered Saline with Tween® 20 (PBS-T) pH 7.4

PBS-T buffer contains the non-ionic detergent Tween TM 20 which acts through blocking. It has the ability to reduce non-specific binding and protein-protein interaction during the wash step in protein and immunoassay procedures such as ELISA and Western blotting. Decreasing the non-specific binding and staining makes ELISA results and blots easier to interpret.

Medicago AB

Catalogue No.DescriptionPack SizePriceQty
MC-09-8902-100Phosphate Buffered Saline with Tween® 20 (PBS-T) pH 7.4, 500 ml 100 tablets £122.00 Quantity Add to Order
MC-09-8902-12Phosphate Buffered Saline with Tween® 20 (PBS-T) pH 7.4, 500 ml 12 tablets £29.00 Quantity Add to Order
MC-09-8903-100Phosphate Buffered Saline with Tween® 20 (PBS-T) pH 7.4, 100 ml 100 tablets £31.00 Quantity Add to Order
MC-09-9410-10Phosphate Buffered Saline with Tween® 20 (PBS-T) pH 7.4, 1000 ml 10 tablets / blister pack £38.00 Quantity Add to Order
MC-09-9410-100Phosphate Buffered Saline with Tween® 20 (PBS-T) pH 7.4, 1000 ml 100 tablets / blister pack £193.00 Quantity Add to Order

Phosphate Buffered Saline with Tween® 20 (PBS-T) pH 7.4

PBS-T buffer contains the non-ionic detergent Tween TM 20 which acts through blocking. It has the ability to reduce non-specific binding and protein-protein interaction during the wash step in protein and immunoassay procedures such as ELISA and Western blotting. Decreasing the non-specific binding and staining makes ELISA results and blots easier to interpret.

Medicago AB

Product description

PBS-T buffer contains the non-ionic detergent Tween TM 20 which acts through blocking. It has the ability to reduce non-specific binding and protein-protein interaction during the wash step in protein and immunoassay procedures such as ELISA and Western blotting. Decreasing the non-specific binding and staining makes ELISA results and blots easier to interpret.

Medicago’s PBS-T buffer is supplied as exactly pre-weighed tablets in bottles and in convenient blister packs. Final buffer volumes range from 100 ml to 1000 ml. One tablet dissolved in deionized water yields a 0.14 M sodium chloride 0.0027 M potassium chloride, 0.05 % TweenTM 20, 0.01 M phosphate buffer with pH 7.4 at 25°C.

Features

  • Formulated from analytical grade reagents
  • PBS with non-ionic detergent
  • Specifically developed for immunoassays
  • Exactly pre-weighed tablets
  • Dissolve and go for greater convenience

 

 

 

Directions for use

Deposit one tablet in a laboratory flask or beaker placed on a magnetic stirrer. Add 50 ml to 300 ml of deionized water and stir the solution for a few minutes. Adjust up to the given final buffer volume, stir until full dissolution and the buffer is ready to use.

 

Shipping and storage

PBS-T buffer is shipped at room temperature. Store the product in a dry place at room temperature. Shelf life is three years after production date.

 

Specifications

Chemicals: Analytical grade

Format: Exactly pre-weighed tablets

Composition: 0.14 M NaCl, 0.0027 M KCl, 0.05% TweenTM 20, 0.010 M PO43-

Volume: 100 ml, 500 ml and 1000 ml

pH: 7.4 ± 0.05 at 25°C

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

Phosphate Buffered Saline with Tween® 20 (PBS-T) pH 7.4

PBS-T buffer contains the non-ionic detergent Tween TM 20 which acts through blocking. It has the ability to reduce non-specific binding and protein-protein interaction during the wash step in protein and immunoassay procedures such as ELISA and Western blotting. Decreasing the non-specific binding and staining makes ELISA results and blots easier to interpret.

Medicago AB

Applications

  • Wash buffer in immunolabelling techniques, such as ELISA and Western blotting
  • Blocking buffer for plate based assays
  • Protein-plate coating

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200