- Selectively reacts with primary aliphatic amine groups
- Useful in preparation of oligos with fluorophores sensitive to basic deprotection conditions
Fluorescein is a bright fluorophore that is utilized in a wide range of
applications and is especially popular for oligonucleotide labeling.
Most syntheses directly incorporate fluorescein using the
phosphoramidite approach, and this NHS ester provides the option for
post-deprotection labeling. When paired with a 5’-amino-modifier C6
(10-1906, 10-1907, 10-1916 or 10-1947), the resulting product would be
identical to that generated with 5’-Fluorescein Phosphoramidite
(10-5901).
Product Specifications
Usage
- Coupling:
For labeling 0.2 µmoles of an amino-modified oligonucleotide, dissolve
2.4 mg of the Fluorescein NHS Ester in 100 µL DMSO (or DMF). Dissolve
the amino-modified oligonucleotide in 200 µL 0.25 M
carbonate/bicarbonate buffer (pH 9). Add 20 µL of the NHS Ester solution
and vortex. Let react for 60 min at room temperature and desalt.
| Diluent | DMF or DMSO |
| Storage | Freezer storage, -10 to -30°C, dry |
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