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Oligo Synthesis

Oligo Synthesis : CEPs

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dSpacer-5’-CE Phosphoramidite

3'-O-Dimethoxytrityl-1',2'-Dideoxyribose-5'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Glen Research

Catalogue No.DescriptionPack SizePriceQty
  • Change to:
  • £
10-4191-02dSpacer-5’-CE Phosphoramidite0.25g €409.20 Quantity Add to Order
10-4191-90dSpacer-5’-CE Phosphoramidite100µmoles €126.00 Quantity Add to Order

Description

In oligonucleotide applications, there is often a need to introduce blocking groups on the 3’-end and sometimes at the 5’-end as well. These blocking groups can provide exonuclease protection or prevent polymerase extension. One such blocking group is an inverted dSpacer, which can be incorporated with dSpacer-5’-CE Phosphoramidite during standard 3’ to 5’ syntheses.

Formula:C35H45N2O6P
M.W.:620.73
F.W.:180.10
CAS Number: 401813-16-9

 

 

Summary: Introducing another option for inverted dSpacer modification and blocking group for 5’ and 3’-end modification

Advantages: Significantly enhances nuclease stability

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References

Download SDS from supplier's website here

Download Glen Report article from supplier's website here

H.J. Haringsma, et al., Nucleic Acids Res, 2012, 40, 4125-36.

Y. Pei, et al., RNA, 2010, 16, 2553-63.

F. Czauderna, et al., Nucleic Acids Res, 2003, 31, 2705-16.

S. Yoon, and J.J. Rossi, Curr Pharm Biotechnol, 2018, 19, 604-610.

T.C. Jarvis, et al., J Biol Chem, 1996, 271, 29107-12.

D.G. Macejak, et al., Hepatology, 2000, 31, 769-76.

L. Beigelman, et al., J Biol Chem, 1995, 270, 25702-8.

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Notes

Coupling

No changes needed from standard method recommended by synthesizer manufacturer.

Deprotection

No changes needed from standard method recommended by synthesizer manufacturer.

Diluent

Anhydrous Acetonitrile

Storage

Freezer storage, -10 to -30°C, dry Stability 2-3 Days

View dilution/coupling data on supplier's website here

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Applications & Benefits

Therapeutic studies (e.g. siRNA, saRNA, hammerhead ribozymes)

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Related products

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