Cambio - Excellence in Molecular Biology

In Vitro Toxicology

In Vitro Toxicology: Mutagenicity kits

The Ames II / Ames MPF Microfluctuation Assays are all modifications of the traditional Ames plate incorporation assay.

The Ames II assay uses the S. typhimurium strains TA98 and TAMix (a mixture of 6 strains, each detecting a specific base-pair substitution), whereas the Ames MPF® kits are available with the strains TA98, TA100, TA1535, TA1537, E.coli WP2 uvrA or E.coli WP2 pKM101.


Benefits of Xenometrix Ames Mutagenicity Assays over traditional Ames Procedures

  • Easy, compact and high throughput liquid format with 384 well plates.

  • Simple detection method with easy "readout" (as compared to the traditional assay)

  • Kits include all five types strains cited in OECD 471 (CoA supplied), quality controlled according to the same guideline 

  • Much less  test compound required.  (10 mg per strain if tested in triplicates, +/- S9, 6 semi-logarithmic compound dilutions)

  • Ames MPF is the preferred system for genotoxic impurities due to its low consumption of compound

  • Significantly less consumption of S9

  • Less environmental pollution due to significantly less "contaminated" waste

  • Less user intervention

  • Download presentation 

Ames II - 10 Sample Kit for Ames Test with 2 Strains

10 Sample Kit when tested in 2 strains (TA98, TAMix), +/- S9, 3 Replicates, 6 Doses, Positive & Negative Controls: Strains, Media, Ampicillin, PB/ß-Naphtoflavone induced rat liver S9, Controls. Not included in the kit: S9 cofactor solutions, microplates.

Xenometrix AG

Catalogue No.DescriptionPack SizePriceQty
E10-213-S2-PAmes II with phenobarbitol induced S9 pos control10 tests £2,259.00 Quantity Add to Order



Ames II is a miniaturized, ready to use Ames Test kit in a liquid microplate format. It includes all reagents necessary to run 10 samples with strains TA98 and TAMix (Mix of TA7001, TA7002, TA7003, TA7004, TA7005, TA7006): 10 vials each of frozen TA98, TAMix, plus Growth -, Exposure -, Reversion Indicator Media, Ampicillin, Positive Controls, lyophilized Post-Mitochondrial Supernatant S9 from Aroclor 1254 induced rat liver. Not included in the kit are plastic ware and S9 Cofactor solutions.

The kits are quality controlled and do not need an inhouse quality control (phenotypes) like the traditional agar plate assay.

Ames II - 10 Sample Kit for Ames Test with 2 Strains

Service analytics for early drug discovery with test compound of 11 mg / strain ( /- S9) with the Ames MII or Ames MPF assay system is available as well under GLP and non GLP conditions, please contact



  • Quality controlled products: all products of the kit
  • Certificate of Analysis: Provided
  • Excel Calculation Sheet to support Data Interpretation
  • Comparison with agar late test: Corresponds to 2 x 480 agar plates.
  • Enough Material to process Strains individually
  • Shipped on dry ice from external Storage in Germany
  • High sensitivity and excellent correlation with agar plate test (see database and publications on this homepage)
  • 4 times less test compound as compared to the agar plate test
  • Less hands-on-time and simultaneous processing of several replicates
  • Automation available - High throughput format
  • 3Rs - 12 fold less consumption of rat liver liver S9, less test animals
  • Less error prone due to easy colorimetric read-out
  • Waste - Significant less plastic waste and thus reduced contaminated waste in environment

Also available

  • S9 Cofactor Kit (Art.No. PCO-0800) for preparation of S9 Mix
  • A10-210-S1-P Ames MPF 98/100 with S9 induced by Aroclor 1254 - shipped at RT
  • A10-210-S2-P Ames 98/100 with S9 induced by PB/ß-Naphtoflavone induced rat liver S9* - shipped at RT
  • Ames MPF 98/100 Aqua for environmental water samples - shipped at RT
  • Ready to use 5 Strain kits Ames MPF Penta 1 and Penta 2 - shipped at RT
  • Individual Reagents, such as rat liver S9

*strongly recommended over Aroclor induced S9, Aroclor being a strong environmental polutant and on the EC list of banned chemicals.


Short Protocol


Stability studies





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