DNA Polymerase I, Large (Klenow) Fragment is a DNA-dependent
polymerase that lacks the 5′→3′ exonuclease activities of E. coli DNA
Polymerase I from which it is derived. The 3′→5′ remains intact to
remove 3’ overhangs, and along with the polymerase activity to fill in
5’ overhangs and create blunt end DNA fragments. The optimal incubation
temperature of the enzyme is 25 °C and uses 10X DNA Polymerase Buffer
A. Heat inactivation of the enzyme is 75 °C for 20 minutes.
Key features
- Forms blunt ends by removing 3’ overhangs or filling in 5’ overhangs
- Used in second-strand cDNA synthesis
- Generates DNA probes using random primers
Product Details
DNA Polymerase I Large (Klenow) Fragment includes Klenow DNA Polymerase at 5 U/µL and 10X DNA Polymerase Buffer A
SDS
Manuals and user guides