Students are presented with a fictional family’s medical history and
must work to make a genetic diagnosis. The family depicted in this lab,
the Robinson family, has two children, one of whom has had an initial
test result indicating possible sickle cell disease.
This hands-on lab activity exposes students to the fundamental
principles of DNA analysis comparing patterns of cleaved DNA by gel
electrophoresis. Students will gain familiarity with DNA separation
techniques and engage in determining DNA fragment size to obtain a
- Techniques utilized: DNA gel electrophoresis.
- Time required: can be completed in one 45 min. class period
- Reagents needed: ‘Sickle Cell Genetics Lab’ reagents kit (included in this product), electrophoresis reagents (available separately)
Suggested skill level: Intended for any
student seeking familiarity with DNA gel electrophoresis, genetic
disease and diagnosis, from middle school to college.
The Sickle Cell Genetics Lab kit contains reagents for 8 lab groups of up to 5 students each (40 students):
- 4 DNA samples for fingerprinting analysis
- 1 tube of DNA molecular weight marker (DNA Ladder)
- Reagents are compatible with blueGel™ or other standard DNA electrophoresis systems
Free miniPCR Learning Lab™ downloads:
Contact firstname.lastname@example.org for answers key.
Microtubes (50), PCR tubes (100),
blueGel™ Tabs agarose tablets (10 pcs. x 0.4 g ea.), 20X TBE buffer (30
mL), GelGreen™ DNA stain, 10,000X (20 µL). NOTE: this add-on item ships
UPS GROUND at no additional charge. contact us for details
If you cannot find the answer to your problem below then please
contact us or telephone 01954 210 200