Cambio Ni-NTA MagBeads
The His-tag is the most widely used affinity tag due to its small size and versatility under native and denaturing conditions, as well as in presence of detergents and many other additives. Magnetic beads are ideal for protein purification from dilute supernatants and for pull-down experiments. The agarose surface of our MagBeads is identical to that of Cambio Agarose, making them an ideal combination for small-scale screening and upscale reactions.
Cambio Ni-NTA MagBeads are ferrimagnetic agarose beads coupled to an NTA chelating ligand, loaded with nickel ions. They are delivered as a 25% suspension.
Do you prefer Ni-IDA? We offer the same high-quality resin with that chelating ligand.
Why Cambio Ni-NTA MagBeads?
- Highest binding capacity on the market (up to 70 mg/mL settled beads).
- Low unspecific binding
- Superior DTT and EDTA stability
- Ideal for purification from dilute solutions, or pulldown experiments
- Also available as Ni-NTA Agarose and prepacked cartridges
Exceptional binding capacity
Cambio Ni-NTA MagBeads show exceptional binding capacity. In a comparative study against equivalent magnetic beads from two market-leading providers, purification of GFP expressed in E. coli was performed with Cambio Ni-NTA MagBeads, and two similar matrices from Competitor G and Competitor Q. Figure 1 shows the SDS-PAGE of the comparison. Total protein collected in 4 elution fractions was 46% higher from Cambio Ni-NTA MagBeads (Cambio, E1-E4) compared to Competitor G, which was conversely higher than the amount obtained with magnetic beads from Competitor Q.
Figure 1. >46% higher yield obtained with Cambio Ni-NTA MagBeads. SDS-PAGE of GFP expressed in E.coli and purified with comparable amounts of Cambio Ni-NTA MagBeads and equivalent, market-leading magnetic beads. Total protein obtained in 4 elution fractions with Cambio Ni-NTA MagBeads was 46% higher (E1-E4, Cambio) than that obtained with magnetic beads from Competitor G (E1-E4, G) and even higher when compared to eluted protein obtained with magnetic beads from Competitor Q (E1-E4, Q).
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