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Enzymes for Molecular Biology

Enzymes for Molecular Biology: Heat labile & Salt Tolerant Enzymes

New range of enzymes for 2019. Our new range of enzymes include novel enzymes specifically designed or engineered to be amazingly tolerant to high salt concentrations, function at very low temperatures and heat inactivated.

Salt-tolerant DNA & RNA Nuclease (heat-labile)

Salt tolerant DNA & RNA Nuclease is a nonspecific endonuclease degrading double- and single-stranded DNA and RNA with optimum activity at high salt concentrations. It is active in the presence of salt making it the enzyme of choice for removing contaminating nucleic acid in protein purification.

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Catalogue No.DescriptionPack SizePriceQty
CA-1729-25KSalt-tolerant DNA & RNA Nuclease (heat-labile)25000 units 25U/µl £330.00 Quantity Add to Order

Salt-tolerant DNA & RNA Nuclease (heat-labile)

Salt tolerant DNA & RNA Nuclease is a nonspecific endonuclease degrading double- and single-stranded DNA and RNA with optimum activity at high salt concentrations. It is active in the presence of salt making it the enzyme of choice for removing contaminating nucleic acid in protein purification.

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Salt-tolerant DNA & RNA Nuclease (heat-labile)

Salt-tolerant DNA & RNA Nuclease is a heat-labile non-specific endonuclease degrading double- and single-stranded DNA and RNA with optimum activity at high salt concentrations. It is active in a variety of buffers and can be easily inactivated by treatment with a reducing agent even at low temperatures. These features make Salt-tolerant DNA & RNA Nuclease particularly useful in the purification and removal of DNA and RNA after protein purification. The end-products after complete digestion consist mainly of oligos around 5 nucleotides.  The enzyme is compatible with a wide range of buffers used in protein purification.

Main advantages of Salt-tolerant DNA & RNA Nuclease

  • Non specific endonuclease
  • Optimum activity at high salt concentration (0.5 M NaCl)
  • Active at low temperatures (20% at 6ºC)
  • Broad pH range

Source

Recombinantly produced in Pichia pastoris.

Activity
Salt-tolerant DNA & RNA Nuclease is highly active in the temperature range 10–40°C. Optimal NaCl-concentration for activity is 0.5 M, working range is 0.25–1.0 M. Mg2 ; (>1 mM) is required for activity. Working pH range is 7.5–9.5, optimal pH is 9.0.

Storage
Minimum shelf life is 2 years at -20°C. Storage at 4°C is possible for at least 6 months. The enzyme tolerates multiple freeze-thaw cycles.

Unit definition

One unit is defined as the amount of Salt-tolerant DNA & RNA Nuclease that will cause a ΔA260 = 1 in 30 minutes at 37°C in a buffer consisting of 25 mM Tris-HCl, pH 8.5 (25°C), 5 mM MgCl2, 0.5 M NaCl and 50 μg/ml calf thymus DNA (D-1501, Sigma).

 

Note:

Inactivation of Salt-tolerant DNA & RNA Nuclease depends on the right combination of inactivation time, inactivation temperature (4–60°C) and concentration of the reducing agent (1–20 mM). If the reducing agent is completely removed after inactivation, trace amounts (<0.01 %) of Salt-tolerant DNA & RNA nuclease may reactivate. To avoid any reactivation, maintain a low concentration of reducing agent, 0.1–0.5 mM DTT or TCEP, alternatively use prolonged incubation times using 10–20 mM TCEP upon inactivation.

 

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

Salt-tolerant DNA & RNA Nuclease (heat-labile)

Salt tolerant DNA & RNA Nuclease is a nonspecific endonuclease degrading double- and single-stranded DNA and RNA with optimum activity at high salt concentrations. It is active in the presence of salt making it the enzyme of choice for removing contaminating nucleic acid in protein purification.

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Instructions for use

To remove nucleic acids from a sample, add Salt-tolerant DNA & RNA Nuclease to a final concentration of 1 U/μl and incubate for 15–30 minutes at 25–37°C.

 

Inactivation


Salt-tolerant DNA & RNA Nuclease is quickly inactivated by addition of reducing agents like TCEP or DTT. TCEP is the recommended reducing agent as it inactivates Salt-tolerant DNA &RNA nuclease more efficiently. Add TCEP to a final concentration of 10 mM and incubate at 30°C for 30 minutes for complete inactivation of the enzyme. Inactivation can also be accomplished at lower temperatures, e.g. by the addition of 10 mM TCEP and storage at 4°C ON.

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200