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Enzymes for Molecular Biology

Enzymes for Molecular Biology: Heat labile & Salt Tolerant Enzymes

New range of enzymes for 2019. Our new range of enzymes include novel enzymes specifically designed or engineered to be amazingly tolerant to high salt concentrations, function at very low temperatures and heat inactivated.

Cryophile Exonuclease I (heat-labile)

Cryophile Exonuclease I is a 3’-5’ exonuclease, specific for single stranded DNA. Cryophile Exonuclease I is active at lower temperatures (= 25°C) and inactivated by 1 min incubation at 80°C, or 15 min at 60°C.

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Catalogue No.DescriptionPack SizePriceQty
CA-1734-25Cryophile Exonuclease I (heat-labile)2500 U (20U/µl) £60.00 Quantity Add to Order
CA-1734-50Cryophile Exonuclease I (heat-labile)50 kU (20U/µl) POA Quantity Add to Order

Cryophile Exonuclease I (heat-labile)

Cryophile Exonuclease I is a 3’-5’ exonuclease, specific for single stranded DNA. Cryophile Exonuclease I is active at lower temperatures (= 25°C) and inactivated by 1 min incubation at 80°C, or 15 min at 60°C.

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Cryophile Exonuclease I is a 3’-5’ exonuclease, specific for single stranded DNA which is readily inactivated by heating (heat-labile). Cryophile Exonuclease I is active at temperatures ≥25°C, and inactivated by 1 min incubation at 80°C, or 15 min at 60°C. Recombinantly produced in an E. coli strain expressing the ExoI gene originating from an Arctic marine bacterium.

Unit definition: One unit catalyzes the release of 10 nmol of acid soluble nucleotides in 30 min at 25°C. Reaction buffer: 20 mM Tris-HCl pH 7.5 at 25°C, 200 mM NaCl, 20 mM MgCl2, 1 mM DTT, 0.01% Triton X-100, 2.5% glycerol and 3H-dATP-labelled DNA (25 ng/µl).

Specific activity: >50 000 U/mg.

Storage buffer: 10 mM Tris-HCl pH 7.5 (at 25°C), 200 mM NaCl, 10 mM MgCl2, 1 mM DTT, 0.01% (v/v) Triton X-100, 50% (v/v) Glycerol.

Storage conditions: -20°C.

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