Cryophile Shrimp Alkaline Phosphatase PCR Cleanup
A quick, easy and reliable enzymatic reagent for PCR cleanup
- Removes excess primers and dNTPs
- Fast 15 minute protocol
- Add directly to PCR product
- 100% Sample Recovery
- Scalable for different reaction sizes
- Easy to automate
- Enzymatic cleanup of PCR products
The Cryophile Shrimp Alkaline Phosphatase PCR Cleanup method utilizes two hydrolytic enzymes, recombinant Shrimp Alkaline Phosphatase (rSAP) and Exonuclease I (Exo I). The combination of these enzymes ensures complete dephosphorylation of dNTPs and degradation of residual primers, enabling sequencing and other downstream applications. The reagents are active in commonly used PCR buffers and eliminates the need for buffer exchange.
The Cryophile Shrimp Alkaline Phosphatase PCR Cleanup components can be added directly into the PCR product, followed by 5 minute incubation at 37°C. Inactivation of both enzymes is achieved in only 10 minutes at 80°C. PCR products are immediately ready for downstream applications without any need for further processing.
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