Cell Information:
Human Umbilical Artery Endothelial Cells (HUAEC) are isolated from normal human umbilical arteries. Arterial endothelial cells have been used as an in vitro model for the study of morphological and ultrastructural changes in the development of atherosclerosis. HUAEC has also been used for the study of endothelial cell-dependent lymphocyte activation and chemoattractant activity.
The Transfection Reagent:
Transfection of primary cells, such as HUAEC, has always been challenging, both technically and financially. The most common systems currently used for molecular delivery to these cells include expensive electroporation instruments and relatively toxic chemical formulations.
Human Umbilical Artery Endothelial Cell (HUAEC) Avalanche™ Transfection Reagent is a new, proprietary solution specifically designed for transfection on HUAEC. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for HUAEC. No other transfection reagents can match the efficiency, convenience, and gentleness of Human Umbilical Artery Endothelial Cell (HUAEC) Avalanche™ Transfection Reagent for transfection on HUAEC. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?
Features:
- Specifically optimized to deliver nucleic acids into HUAEC
- Highest efficiency to ensure experimental success
- Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
- Deliver single or multiple plasmids
- synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cells lines
- Simplest and straightforward protocols
- Easy-to-use protocol: Just mix nucleic acids with the reagent to form complexes and simply add the mixture to cells
- Compatible with transfection in any plate formats
- Economical: High efficiency means less amount of nucleic acid & reagent is needed
- Developed and manufactured by EZ Biosystems
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| Data: |
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| FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on human aortic endothelial cell after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for human aortic endothelial cell. This test result was confirmed with repeat experiments. The candidate formulas were also tested in human umbilical artery endothelial cell (HUAEC) by tranfecting GFP expression plasmid, and got the same high transfection efficiency after flow cytometry analysis. The one that showed the optimal balance of potent & low cytotoxicity (percentage of 7AAD positive cells) among those candidate formulas was later named as Human Umbilical Artery Endothelial Cell (HUAEC) Avalanche Transfection Reagent. |
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For Other Cells: |
Human Umbilical Artery Endothelial Cell (HUAEC) Avalanche™ Transfection Reagent can also be used on the following cells with high transfection efficiencies.
Human Umbilical Vein Endothelial Cell (HUVEC)
Human Dermal Microvascular Endothelial Cell (HMVEC)
Human Dermal Lymphatic Microvascular Endothelial Cell (HDLMVEC)
Human Artery Endothelial Cell
Human Lung Microvascular Endothelial Cell (HLMVEC, HMVEC-L)
786-O Cell
Caki-1 Cell
MDCK Cell
Vero Cell
293 Cell
293T/17 Cell
Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list. |
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