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Molecular Cloning Kits

Molecular Cloning Kits: Cloning Kits

Fast-Link™ DNA Ligation Kit

The Fast-Link DNA Ligation Kits are specially formulated to provide the fastest high efficiency DNA ligations for routine and high throughput DNA cloning

BioSearch Tech (Lucigen/Epicentre)

Catalogue No.DescriptionPack SizePriceQty
LK0750HFast-Link™ DNA Ligation Kits50 ligations £152.00 Quantity Add to Order
LK6201HFast-Link™ DNA Ligation Kits100 ligations £235.00 Quantity Add to Order

Fast-Link™ DNA Ligation Kit

The Fast-Link DNA Ligation Kits are specially formulated to provide the fastest high efficiency DNA ligations for routine and high throughput DNA cloning

BioSearch Tech (Lucigen/Epicentre)

  • Clone with speed and efficiency
  • Transform into E. coli without clean-up or desalting

Applications

  • Blunt-end and TA cloning of PCR products.
  • Ligation of next-gen sequencing adaptors to blunt-ended DNA.
  • Genomic DNA cloning and subcloning.
  • BAC/fosmid library construction.
  • cDNA cloning.
  • Linker ligation.
The Fast-Link™ DNA Ligation Kit uses a high-quality ligase (Fast-Link T4 DNA Ligase), that was cloned at Epicentre and formulated to provide extremely rapid, high-efficiency DNA ligation. Cohesive-end ligations can be performed in 5 minutes at room temperature. In contrast to other ligases, it is not necessary to desalt Fast-Link ligation reactions prior to transformation of electrocompetent or chemically competent cells. The Fast-Link Kit can be used for routine and high-throughput DNA cloning.
Type of End Ligation Time % White Colonies Recombinants per µg DNA
Overhang 5 min. 93 2.0 x 106
Blunt 15 min. 71 4.4 x 105
PCR product with 3´-A overhang 1 hr. 68 1.2 x 104

Table 1. Fast-Link™ representative results.

Benefits

  • Cohesive-end ligations in 5 minutes at room temperature (Fig. 1).
  • Blunt-end ligations in 15 minutes at room temperature (Fig. 2).
  • Ligation of PCR product with A-overhangs in 1 hour at 16°C.
  • High ligation efficiency (Table 1).
  • Desalting of ligation products is not needed prior to transformation of electrocompetent or chemically competent cells.
  • BAC ligation with inserts >100 kb can be performed in 4 hours, instead of overnight reactions as with other ligases.
Figure 1. Time course for cohesive end ligation using the Fast-Link™ Kit. Lambda Hind III markers were ligated using 2 units of Fast-Link DNA Ligase (Lanes 2-6). Lane M, Kilobase ladder; lane 1, no enzyme.   Figure 2. Time course for blunt-end ligation using the Fast-Link™ Kit. pUC 19 DNA digested with Pvu II was ligated using 2 units of Fast-Link DNA Ligase (lanes 1-6). Lanes M, Kilobase ladder.

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

Fast-Link™ DNA Ligation Kit

The Fast-Link DNA Ligation Kits are specially formulated to provide the fastest high efficiency DNA ligations for routine and high throughput DNA cloning

BioSearch Tech (Lucigen/Epicentre)

Protocols for: Fast-Link™ DNA Ligation Kit

Due to the constant updating of the protocols by the manufacturer we have provided a direct link to Lucigen’s product page, where the latest protocol is available.
Please note this will open a new page or window on your computer.
 
(catalogue number LK0750H / LK6201H)
Please note: all protocols off site are the responsibility of the products supplier

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

Fast-Link™ DNA Ligation Kit

The Fast-Link DNA Ligation Kits are specially formulated to provide the fastest high efficiency DNA ligations for routine and high throughput DNA cloning

BioSearch Tech (Lucigen/Epicentre)

References:

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If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

Fast-Link™ DNA Ligation Kit

The Fast-Link DNA Ligation Kits are specially formulated to provide the fastest high efficiency DNA ligations for routine and high throughput DNA cloning

BioSearch Tech (Lucigen/Epicentre)

Applications

  • Blunt-end and TA cloning of PCR products.
  • Ligation of next-gen sequencing adaptors to blunt-ended DNA.
  • Genomic DNA cloning and subcloning.
  • BAC/fosmid library construction.
  • cDNA cloning.
  • Linker ligation.

Benefits

  • Cohesive-end ligations in 5 minutes at room temperature .
  • Blunt-end ligations in 15 minutes at room temperature .
  • Ligation of PCR product with A-overhangs in 1 hour at 16°C.
  • High ligation efficiency.
  • Desalting of ligation products is not needed prior to transformation of electrocompetent or chemically competent cells.
  • BAC ligation with inserts >100 kb can be performed in 4 hours, instead of overnight reactions as with other ligases.

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

Fast-Link™ DNA Ligation Kit

The Fast-Link DNA Ligation Kits are specially formulated to provide the fastest high efficiency DNA ligations for routine and high throughput DNA cloning

BioSearch Tech (Lucigen/Epicentre)

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200