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Enzymes for Molecular Biology

Enzymes for Molecular Biology: Enzymes for Molecular Biology

Exo-Minus Klenow DNA Polymerase

Klenow DNA Polymerase Exo- is a DNA-dependent DNA polymerase that lacks both the 5'-3' and 3'-5' exonuclease activities of E. coli DNA Polymerase I from which it is derived.

BioSearch Tech (Lucigen/Epicentre)

Catalogue No.DescriptionPack SizePriceQty
KL11101KExo-Minus Klenow DNA Polymerase (D355A, E357A)1kU 10U/ul £195.00 Quantity Add to Order

Exo-Minus Klenow DNA Polymerase

Klenow DNA Polymerase Exo- is a DNA-dependent DNA polymerase that lacks both the 5'-3' and 3'-5' exonuclease activities of E. coli DNA Polymerase I from which it is derived.

BioSearch Tech (Lucigen/Epicentre)

Klenow DNA Polymerase Exo- is a DNA-dependent DNA polymerase that lacks both the 5'- 3' and 3'- 5' exonuclease activities of E. coli DNA Polymerase I from which it is derived. 

Concentration and Specific Activity

20 Units /µl at greater than 1 X 104 Units/mg 

Unit Definition

One Unit of Klenow DNA Polymerase Exo- converts 10 nmoles of dNTPs into acid-insoluble material in 30 minutes at 37°C. 

Quality Control

Klenow DNA Polymerase Exo- is free of detectable endo- and exonuclease and RNase activities.



If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

Exo-Minus Klenow DNA Polymerase

Klenow DNA Polymerase Exo- is a DNA-dependent DNA polymerase that lacks both the 5'-3' and 3'-5' exonuclease activities of E. coli DNA Polymerase I from which it is derived.

BioSearch Tech (Lucigen/Epicentre)

Protocols for: Klenow DNA Polymerase Fragment Exo-

 

Due to the constant updating of the protocols by the manufacturer we have provided a direct link to Lucigen’s product page, where the latest protocol is available.

Please note this will open a new page or window on your computer.

 

 

Klenow DNA Polymerase Fragment Exo- Protocol

(catalogue number KL0810250/ KL081001K/ KL105000/ KL080911K)

Please note: all protocols off site are the responsibility of the products supplier

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

Exo-Minus Klenow DNA Polymerase

Klenow DNA Polymerase Exo- is a DNA-dependent DNA polymerase that lacks both the 5'-3' and 3'-5' exonuclease activities of E. coli DNA Polymerase I from which it is derived.

BioSearch Tech (Lucigen/Epicentre)

References

 

    1. Feinberg, A.P. and Vogelstein, B. (1984) Anal. Biochem. 137, 266.

 

    1. Sanger, F. et. al. (1977) Proc. Natl. Acad. Sci. USA 74, 5463.

 

    1. Molecular Cloning: A Laboratory Manual (1989), J. Sambrook, E.F. Fritsch and T. Maniatis, editors, Cold Spring Harbor Laboratory Press, 2nd edition, v.2, p. 8.14.

 

  1. Walter, N.G. (1995) J. Mol. Biol. 254, 856.

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

Exo-Minus Klenow DNA Polymerase

Klenow DNA Polymerase Exo- is a DNA-dependent DNA polymerase that lacks both the 5'-3' and 3'-5' exonuclease activities of E. coli DNA Polymerase I from which it is derived.

BioSearch Tech (Lucigen/Epicentre)

Applications

  • Random-primer labeling of DNA.1
  • Second-strand cDNA synthesis.2
  • Strand-displacement amplification.3

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200