Cambio - Excellence in Molecular Biology

Enzymes for Molecular Biology

Enzymes for Molecular Biology: Enzymes for Molecular Biology

Rec J Exonuclease

Rec J Exonuclease, derived from E. coli, catalyzes degradation of ssDNA in a 5´?3´ direction and is used for the removal of primers from completed PCR and degradation of single-stranded linear DNA in dsDNA and plasmid preps.

BioSearch Tech (Lucigen/Epicentre)

Catalogue No.DescriptionPack SizePriceQty
RJ411250Rec J Exonuclease250U 10 U/µl £231.00 Quantity Add to Order

Rec J Exonuclease

Rec J Exonuclease, derived from E. coli, catalyzes degradation of ssDNA in a 5´?3´ direction and is used for the removal of primers from completed PCR and degradation of single-stranded linear DNA in dsDNA and plasmid preps.

BioSearch Tech (Lucigen/Epicentre)

Rec J Exonuclease, derived from E. coli, catalyzes removal of deoxyribonucleoside monophosphates from single-stranded DNA in a 5'- 3' direction. Its activity is dependent on Mg 2. Rec J Exonuclease can be heat-inactivated by incubation at 65°C for 20 minutes. 

Unit Definition

One unit is the amount of enzyme that catalyzes release of 1 nmol of acid-soluble nucleotides from activated single-stranded calf thymus DNA in 30 minutes at 37°C. 

Storage Buffer

50% glycerol containing 50 mM Tris-HCl (pH 7.5), 0.1 mM EDTA, 1 mM DTT, 0.1 M NaCl, and 0.1%Triton® X-100. 

10X Reaction Buffer

330 mM Tris-acetate (pH 7.8), 660 mM potassium acetate, 100 mM magnesium, acetate, and 5.0 mM DTT. 

Quality Control

Rec J Exonuclease is free of detectable RNase and contaminating DNA exo- and endonuclease activities.



If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

Rec J Exonuclease

Rec J Exonuclease, derived from E. coli, catalyzes degradation of ssDNA in a 5´?3´ direction and is used for the removal of primers from completed PCR and degradation of single-stranded linear DNA in dsDNA and plasmid preps.

BioSearch Tech (Lucigen/Epicentre)

Protocols for: Rec J Exonuclease, E. coli

Rec J Exonuclease Protocol

(catalogue number RJ411050 / RJ411250) 

Please note: all protocols off site are the responsibility of the products supplier

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

Rec J Exonuclease

Rec J Exonuclease, derived from E. coli, catalyzes degradation of ssDNA in a 5´?3´ direction and is used for the removal of primers from completed PCR and degradation of single-stranded linear DNA in dsDNA and plasmid preps.

BioSearch Tech (Lucigen/Epicentre)

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If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

Rec J Exonuclease

Rec J Exonuclease, derived from E. coli, catalyzes degradation of ssDNA in a 5´?3´ direction and is used for the removal of primers from completed PCR and degradation of single-stranded linear DNA in dsDNA and plasmid preps.

BioSearch Tech (Lucigen/Epicentre)

Applications

  • Removal of primers from completed PCR.
  • Degradation of single-stranded linear DNA in dsDNA and plasmid preps

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200