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Enzymes for Molecular Biology

Enzymes for Molecular Biology: Enzymes for Molecular Biology

RNase A

Ribonuclease A (RNase A) is an endoribonuclease that cleaves single-stranded RNA at the 3'-end of pyrimidine residues

BioSearch Tech (Lucigen/Epicentre)

Catalogue No.DescriptionPack SizePriceQty
MRNA092RNase A5 µg/µl 2 ml £63.00 Quantity Add to Order

RNase A

Ribonuclease A (RNase A) is an endoribonuclease that cleaves single-stranded RNA at the 3'-end of pyrimidine residues

BioSearch Tech (Lucigen/Epicentre)

Ribonuclease A (RNase A) is an endoribonuclease that cleaves single-stranded RNA at the 3'-end of pyrimidine residues, forming oligoribonucleotides having 3'-terminal pyrimidine-3'-phosphates. Pyrimidine-3'-monophosphates are also released by RNase A cleavage of adjacent pyrimidine nucleotides.

Modified RNA containing pyrimidine-2'-fluoro-dNMPs, such as modified RNA made by in vitro transcription using the DuraScribe® T7 & SP6 Transcription Kits is completely resistant to cleavage by RNase A. 

Composition and Concentration

Provided on the basis of weight at 5 mg/ml in Storage Buffer

Storage Buffer

50% glycerol containing 50 mM Tris-HCl (pH 7.5), 0.1 mM EDTA, 1 mM DDT, 0.1 M NaCl, and 0.1% Triton® X-100

Quality Control

RNase A is free of contaminating DNA exo- and endonuclease activities   

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

RNase A

Ribonuclease A (RNase A) is an endoribonuclease that cleaves single-stranded RNA at the 3'-end of pyrimidine residues

BioSearch Tech (Lucigen/Epicentre)

protocol

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

RNase A

Ribonuclease A (RNase A) is an endoribonuclease that cleaves single-stranded RNA at the 3'-end of pyrimidine residues

BioSearch Tech (Lucigen/Epicentre)

References

  1. Johnson, M. (1996) EPICENTRE Forum 3(4), 7.
  2. Shen, V. and Schlessinger, D. (1982) The Enzymes XV (Part B), 501.
  3. delCardayré, S.B. and Raines, R.T. (1995) Anal. Biochem. 225, 176.

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

RNase A

Ribonuclease A (RNase A) is an endoribonuclease that cleaves single-stranded RNA at the 3'-end of pyrimidine residues

BioSearch Tech (Lucigen/Epicentre)

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If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

RNase A

Ribonuclease A (RNase A) is an endoribonuclease that cleaves single-stranded RNA at the 3'-end of pyrimidine residues

BioSearch Tech (Lucigen/Epicentre)

Applications

  • Removal of RNA from DNA preparations.1
  • RNase protection assays to detect single-basepair mismatches in RNA:RNA and RNA:DNA hybrids.1

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200