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Enzymes for Molecular Biology

Enzymes for Molecular Biology: Enzymes for Molecular Biology

RNA 5' Polyphosphatase

RNA 5' Polyphosphatase is used for the Conversion of 5´-triphosphorylated RNA to 5´-monophosphorylated RNA for use in 5´-RNA ligation-tagging methods using T4 RNA Ligase, analysis of 5´-end structure of RNA and preparation of substrate RNA molecules for subsequent degradation using EPICENTRE's Terminator™ 5´-Phosphate-Dependent Exonuclease.

BioSearch Tech (Lucigen/Epicentre)

Catalogue No.DescriptionPack SizePriceQty
RP8092H RNA 5' Polyphosphatase20 U/µl £105.00 Quantity Add to Order

RNA 5' Polyphosphatase

RNA 5' Polyphosphatase is used for the Conversion of 5´-triphosphorylated RNA to 5´-monophosphorylated RNA for use in 5´-RNA ligation-tagging methods using T4 RNA Ligase, analysis of 5´-end structure of RNA and preparation of substrate RNA molecules for subsequent degradation using EPICENTRE's Terminator™ 5´-Phosphate-Dependent Exonuclease.

BioSearch Tech (Lucigen/Epicentre)

RNA 5' Polyphosphatase* is a Mg2 -independent phosphohydrolase discovered and characterized by EPICENTRE scientists. The enzyme sequentially removes the γ and β phosphates from 5´-triphosphorylated RNA (such as primary RNA transcripts):
5´ pppN—OH 3' → 5' pN—OH 3´ 2 Pi 

RNAs with a 5´-diphosphorylated end are also converted to 5'-monophosphorylated RNA by RNA 5' Polyphosphatase:
5' ppN—OH 3' → 5' pN—OH 3' Pi 

RNA Polyphosphatase has no activity on RNA with a 5' cap (e.g., 5' m7GpppN—OH 3'), or a 5'-monophosphorylated end (5' pN—OH 3'). However, both NTPs and dNTPs are substrates for the enzyme, yielding the corresponding NMPs and dNMPs inorganic phosphate: (d)NTP → (d)NMP 2Pi 

Unit Definition

One unit of RNA 5' Polyphosphatase releases 1 nmol of inorganic phosphate from ATP in 1 hour at 37°C under standard assay conditions. 

Storage Buffer

50% glycerol containing 0.05 M Tris-HCl (pH 7.5), 0.1 mM EDTA, 1 mM DTT, 0.1 M NaCl, and 0.1% Triton® X-100. 

10X Reaction Buffer

0.5 M HEPES-KOH (pH 7.5), 1 M NaCl, 10 mM EDTA, 1% β-mercaptoethanol, and 0.1% Triton® X-100. 

Inactivation/Inhibitors

RNA 5' Polyphosphatase is inhibited ~50% by 100 mM of inorganic phosphate (Pi) using ATP as a substrate. 

Quality Control

RNA 5' Polyphosphatase is free of detectable DNA exo- and endonuclease, and RNase activities.

 *Covered by issued and/or pending patents.

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

RNA 5' Polyphosphatase

RNA 5' Polyphosphatase is used for the Conversion of 5´-triphosphorylated RNA to 5´-monophosphorylated RNA for use in 5´-RNA ligation-tagging methods using T4 RNA Ligase, analysis of 5´-end structure of RNA and preparation of substrate RNA molecules for subsequent degradation using EPICENTRE's Terminator™ 5´-Phosphate-Dependent Exonuclease.

BioSearch Tech (Lucigen/Epicentre)

Protocols

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Please note: all protocols off site are the responsibility of the products supplier

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

RNA 5' Polyphosphatase

RNA 5' Polyphosphatase is used for the Conversion of 5´-triphosphorylated RNA to 5´-monophosphorylated RNA for use in 5´-RNA ligation-tagging methods using T4 RNA Ligase, analysis of 5´-end structure of RNA and preparation of substrate RNA molecules for subsequent degradation using EPICENTRE's Terminator™ 5´-Phosphate-Dependent Exonuclease.

BioSearch Tech (Lucigen/Epicentre)

Citations for RNA 5´ Polyphosphatase

  1. Fujimura, T. & Esteban, R. (2010) Yeast Double-stranded RNA Virus L-A Deliberately Synthesizes RNA Transcripts with 5'-Diphosphate, J. Biol. Chem. 285 , 22911-22918.

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

RNA 5' Polyphosphatase

RNA 5' Polyphosphatase is used for the Conversion of 5´-triphosphorylated RNA to 5´-monophosphorylated RNA for use in 5´-RNA ligation-tagging methods using T4 RNA Ligase, analysis of 5´-end structure of RNA and preparation of substrate RNA molecules for subsequent degradation using EPICENTRE's Terminator™ 5´-Phosphate-Dependent Exonuclease.

BioSearch Tech (Lucigen/Epicentre)

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If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

RNA 5' Polyphosphatase

RNA 5' Polyphosphatase is used for the Conversion of 5´-triphosphorylated RNA to 5´-monophosphorylated RNA for use in 5´-RNA ligation-tagging methods using T4 RNA Ligase, analysis of 5´-end structure of RNA and preparation of substrate RNA molecules for subsequent degradation using EPICENTRE's Terminator™ 5´-Phosphate-Dependent Exonuclease.

BioSearch Tech (Lucigen/Epicentre)

Applications

    • Conversion of 5'-triphosphorylated RNA to 5'-monophosphorylated RNA for use in 5'-RNA ligation-tagging methods using T4 RNA Ligase.
    • Analysis of 5'-end structure of RNA.
    • Preparation of substrate RNA molecules for subsequent degradation using EPICENTRE's Terminator™ 5'-Phosphate-Dependent Exonuclease.

 

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200