Rapid quantification of rare target sequences in dilute samples
TATAA One-Step Fast GrandMaster® Kit is a 4X concentrated fastmix
which allows analysis of larger volumes of sample. This results in an
ability to quantify rare target sequences in dilute samples.
One-Step Fast GrandMaster® Kit has been developed for fast and highly
sensitive qRT-PCR of a wide range of RNA targets. It is optimized for
use with conventional 5’ hydrolysis probes and allows for unbiased
amplification of low copy transcripts in the presence of high copy
- TATAA One-Step Reverse Transcriptase (20x)
- TATAA One-Step Fast GrandMaster® Mix (4x): 4x reaction buffer
containing optimised concentrations of MgCl2, dNTPs, Taq DNApolymerase
- Nuclease-free water
The TATAA SYBR® GrandMaster® Mix can be ordered without ROX, with
low ROX or with ROX, depending on the instrument used for the analysis.
The number of reactions is based on 20μl qPCR-reactions.
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