Our original Universal Support (20-5000) has been discontinued since complete dephosphorylation using ammonium hydroxide, AMA and anhydrous methylamine gas takes longer than most companies wish to allocate. A recent development has been the use of a support based on a molecule which is “conformationally preorganized” to accelerate the dephosphorylation reaction.1,2 By using a rigid bicyclic molecule on the support, it was hoped that the molecule’s conformation would facilitate the formation of the cyclic phosphate transition state, thereby stimulating the rate of dephosphorylation. It was found that the rate of elimination is markedly faster than the original Universal Support.
The structure of Glen UnySupport is shown below. The N-phenyl version, developed at Isis Pharmaceuticals as UnyLinker™, is available from several companies for large scale oligo synthesis. Glen UnySupport is the N-methyl version, which is preferred for high throughput oligonucleotide synthesis since methylamine rather than aniline is formed on deprotection. We are happy to offer Glen UnySupport in a variety of popular formats under license from Isis Pharmaceuticals.
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