6-Fluorescein Serinol Phosphoramidite |
Diluent: Anhydrous Acetonitrile
Coupling: 12-15 minute coupling time recommended.
Deprotection: Use ammonium hydroxide and deprotect as required by nucleobases. When using AMA, a small amount of a non-fluorescent impurity will be formed. To eliminate this impurity, first deprotect with ammonium hydroxide for 30 minutes at room temperature, add an equal volume of 40% methylamine and then complete the deprotection as required by the nucleobases - e.g. 10 minutes at 65°C or 2 hours at room temperature for standard bases.
Storage: Freezer storage, -10 to -30°C, dry
Stability in Solution: 2-3 days
Formula: C67H75N4O14P
M.W.: 1191.3
F.W.: 582.45
Please Note: This product is covered by US Patent No.: 8,394,948.
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