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Oligo Synthesis

Oligo Synthesis : CEPs

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Carboxy-dT

Carboxy-dT

Glen Research

Catalogue No.DescriptionPack SizePriceQty
10-1035-02Carboxy-dT 0.25g £382.00 Quantity Add to Order
10-1035-90Carboxy-dT 100µmoles £182.00 Quantity Add to Order

Description

Carboxy-dT

Structure

Catalog Number: 10-1035-xx

Description: Carboxy-dT

5'-Dimethoxytrityl-5-[3-methyl-acrylate]-2'-deoxyUridine,3'-[(2-cyanoethyl)-
(N,N-diisopropyl)]-phosphoramidite
Formula: C43H51N4O10P M.W.: 814.88 F.W.: 360.22
Diluent: Anhydrous Acetonitrile
Coupling: No changes needed from standard method recommended by synthesizer manufacturer.
Deprotection: Cleavage and deprotection should be carried out using a mild deprotection: 0.4M methanolic sodium hydroxide (methanol:water 4:1) for 17 hours at room temperature. Pipet off support and neutralize with 2M TEAA.Note: NaOH is not compatible with dmf protecting groups.
Storage: Refrigerated storage, maximum of 2-8°C, dry

Stability in Solution: 2-3 days

SEQUENCE MODIFIERS

Sequence Modifiers are designed for use in automated synthesis. The carboxy-dT is hydrolyzed during deprotection and can be coupled directly to a molecule containing a primary amino group by a standard peptide coupling or via the intermediate N-hydroxysuccinimide (NHS) ester. Amino-Modifier dA, Amino-Modifier dC, Amino-Modifier dG and both Amino-Modifier dT products can be added in place of a dA, dC, dG and dT residue, respectively, during oligonucleotide synthesis. Corresponding Amino-Modifier supports can replace their respective deoxynucleoside supports. After deprotection, the primary amine on the C6 analogues is separated from the oligonucleotide by a spacer arm with a total of 7 -10 atoms and can be labelled or attached to an enzyme. The C2 analogue is more suitable for the attachment of molecules designed to react with the oligonucleotide.

 

 

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Protocols

Material Safety Data Sheet

Glen Report 6.1: DEOXYURIDINE DERIVATIVES FOR INTERNAL MODIFICATION

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Applications & Benefits

EXTINCTION DATA

Item Nucleoside λMax-1 Emax-1 λMax-2 Emax-2 E260


(nm) (ml/µmole) nm (ml/µmole) (ml/µmole)
10-1035 Carboxy-dT 297 16.1 261 14.7 14.7

DILUTION/COUPLING DATA

The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.

ABI 392/394
Cat.No. Pack
Size		 Grams/
Pack		 0.1M Dil.
(mL)		 LV40 LV200 40nm 0.2µm 1µm 10µm
Approximate Number of Additions
10-1035-02 0.25grams .25grams 3.07 89 53.4 33.38 24.27 17.8 4.45
10-1035-90 100µmoles .081grams 1 20 12 7.5 5.45 4 1
Expedite
Cat.No. Pack
Size		 Grams/
Pack		 Dilution
(mL)		 Molarity 50nm 0.2µm 1µm 15µm
Approximate Number of Additions
10-1035-02 0.25grams .25grams 4.58 .07 85.2 53.25 38.73 5.33
10-1035-90 100µmoles .081grams 1.5 .07 23.6 14.75 10.73 1.48
Beckman
Cat.No. Pack
Size		 Grams/
Pack		 Dilution
(mL)		 Molarity 30nm 200nm 1000nm

Approximate Number of Additions
10-1035-02 0.25grams .25grams 4.58 .07 86.8 54.25 39.45

10-1035-90 100µmoles .081grams 1.5 .07 25.2 15.75 11.45

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Related products

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