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Oligo Synthesis

Oligo Synthesis : CEPs

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dSpacer CE Phosphoramidite

dSpacer CE Phosphoramidite

Glen Research

Catalogue No.DescriptionPack SizePriceQty
10-1914-02dSpacer CE Phosphoramidite 0.25g £341.00 Quantity Add to Order
10-1914-90dSpacer CE Phosphoramidite 100µmoles £105.00 Quantity Add to Order

Description

dSpacer CE Phosphoramidite

Structure

Catalog Number: 10-1914-xx

Description: dSpacer CE Phosphoramidite

5'-O-Dimethoxytrityl-1',2'-Dideoxyribose-3'-[(2-cyanoethyl)-
(N,N-diisopropyl)]-phosphoramidite
Formula: C35H45N2O6P M.W.: 620.73 F.W.: 180.10
Diluent: Anhydrous Acetonitrile
Add fresh diluent to product vial to recommended concentration and swirl vial occasionally over several minutes until product is completely dissolved. (Some oils may require between 5 and 10 minutes.) Use care to maintain anhydrous conditions. In case of transfer to alternate vial type, ensure recipient vial has been pre-dried. For more information, see http://www.glenres.com/Technical/TB_ABITransfer.pdf.
Coupling: No changes needed from standard method recommended by synthesizer manufacturer.
Deprotection: No changes needed from standard method recommended by synthesizer manufacturer.
Storage: Freezer storage, -10 to -30°C, dry
Stability in Solution: 2-3 days

SPACER MODIFIERS

The spacer phosphoramidites C3, 9, C12 and 18 are used to insert a spacer arm in an oligonucleotide. The compounds may be added in multiple additions when a longer spacer is required. 3’-Spacer C3 CPG may also act as a blocker of exonuclease and polymerase activity at the 3’-terminus. dSpacer is used to introduce a stable abasic site within an oligonucleotide. PC Spacer is a photocleavable C3 spacer modifier, part of our line of photocleavable (PC) modifiers

SPACER MODIFIERS

The spacer phosphoramidites C3, 9, C12 and 18 are used to insert a spacer arm in an oligonucleotide. The compounds may be added in multiple additions when a longer spacer is required. 3’-Spacer C3 CPG may also act as a blocker of exonuclease and polymerase activity at the 3’-terminus. dSpacer is used to introduce a stable abasic site within an oligonucleotide. PC Spacer is a photocleavable C3 spacer modifier, part of our line of photocleavable (PC) modifiers

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Protocols

Material Safety Data Sheet

Glen Report 7.1: NEW MODIFICATION REAGENTS AND SUPPORTS

Glen Report 16.1: Minor Base and Related Novel Phosphoramidites

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References

(1) M. Salunkhe, T.F. Wu, and R.L. Letsinger, J. Amer. Chem. Soc., 1992, 114, 8768-8772.
(2) N.G. Dolinnaya, M. Blumenfeld, I.N. Merenkova, T.S. Oretskaya, N.F. Krynetskaya, M.G. Ivanovskaya, M. Vasseur, and Z.A. Shabarova, Nucleic Acids Res., 1993, 21, 5403-5407.
(3) M. Takeshita, C.N. Chang, F. Johnson, S. Will, and A.P. Grollman, J. Biol. Chem., 1987, 262, 10171-10179.
(4) M.W. Kalnik, C.N. Chang, A.P. Grollman, and D.J. Patel, Biochemistry, 1988, 27, 924-931.
(5) H. Urata and M. Akagi, Tetrahedron Lett., 1993, 34, 4015-4018.
(6) C.J. Marasco, N.J. Angelino, B. Paul, and B.J. Dolnick, Tetrahedron Lett., 1994, 35, 3029-3032.

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Notes

dSpacer CE Phosphoramidite

5'-O-Dimethoxytrityl-1',2'-Dideoxyribose-3'-[(2-cyanoethyl)-
(N,N-diisopropyl)]-phosphoramidite
Formula: C35H45N2O6P M.W.: 620.73 F.W.: 180.10
Diluent: Anhydrous Acetonitrile
Add fresh diluent to product vial to recommended concentration and swirl vial occasionally over several minutes until product is completely dissolved. (Some oils may require between 5 and 10 minutes.) Use care to maintain anhydrous conditions. In case of transfer to alternate vial type, ensure recipient vial has been pre-dried. For more information, see http://www.glenres.com/Technical/TB_ABITransfer.pdf.
Coupling: No changes needed from standard method recommended by synthesizer manufacturer.
Deprotection: No changes needed from standard method recommended by synthesizer manufacturer.
Storage: Freezer storage, -10 to -30°C, dry
Stability in Solution: 2-3 days
Results in stable abasic sites in oligonucleotides.

Often called abasic furan or ab furan
  Material Safety Data Sheet

Literature Highlights

Glen Report 7.1: NEW MODIFICATION REAGENTS AND SUPPORTS
Glen Report 16.1: Minor Base and Related Novel Phosphoramidites

DILUTION/COUPLING DATA

The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.

ABI 392/394
Cat.No. Pack
Size		 Grams/
Pack		 0.1M Dil.
(mL)		 LV40 LV200 40nm 0.2µm 1µm 10µm
Approximate Number of Additions
10-1914-02 0.25grams .25grams 4.03 121 72.6 45.38 33 24.2 6.05
10-1914-90 100µmoles .062grams 1 20 12 7.5 5.45 4 1
Expedite
Cat.No. Pack
Size		 Grams/
Pack		 Dilution
(mL)		 Molarity 50nm 0.2µm 1µm 15µm  
Approximate Number of Additions
10-1914-02 0.25grams .25grams 6.01 .067 113.8 71.13 51.73 7.11  
10-1914-90 100µmoles .062grams 1.5 .067 23.6 14.75 10.73 1.48  
Beckman
Cat.No. Pack
Size		 Grams/
Pack		 Dilution
(mL)		 Molarity 30nm 200nm 1000nm    
Approximate Number of Additions
10-1914-02 0.25grams .25grams 6.01 .067 115.4 72.13 52.45    
10-1914-90 100µmoles .062grams 1.5 .067 25.2 15.75 11.45

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Applications & Benefits

DILUTION/COUPLING DATA

The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.


ABI 392/394
Cat.No. Pack
Size		 Grams/
Pack		 0.1M Dil.
(mL)		 LV40 LV200 40nm 0.2µm 1µm 10µm
Approximate Number of Additions
10-1914-02 0.25grams .25grams 4.03 121 72.6 45.38 33 24.2 6.05
10-1914-90 100µmoles .062grams 1 20 12 7.5 5.45 4 1
Expedite
Cat.No. Pack
Size		 Grams/
Pack		 Dilution
(mL)		 Molarity 50nm 0.2µm 1µm 15µm
Approximate Number of Additions
10-1914-02 0.25grams .25grams 6.01 .07 113.8 71.13 51.73 7.11
10-1914-90 100µmoles .062grams 1.5 .07 23.6 14.75 10.73 1.48
Beckman
Cat.No. Pack
Size		 Grams/
Pack		 Dilution
(mL)		 Molarity 30nm 200nm 1000nm

Approximate Number of Additions
10-1914-02 0.25grams .25grams 6.01 .07 115.4 72.13 52.45

10-1914-90 100µmoles .062grams 1.5 .07 25.2 15.75 11.45

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200

Related products

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