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Oligo Synthesis

Oligo Synthesis : Supports, CPGs

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Epoch EclipseTM Quencher CPG

Epoch EclipseTM Quencher CPG

Glen Research

Catalogue No.DescriptionPack SizePriceQty
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20-5925-01Epoch EclipseTM Quencher CPG 0.1g €327.60 Quantity Add to Order
20-5925-10Epoch EclipseTM Quencher CPG 1.0g €3,006.00 Quantity Add to Order
20-5925-13Epoch EclipseTM Quencher CPG 1x10µm €1,086.00 Quantity Add to Order
20-5925-14Epoch EclipseTM Quencher CPG 1x15µm €1,630.80 Quantity Add to Order
20-5925-41Epoch EclipseTM Quencher CPG 4x1.0µm €381.60 Quantity Add to Order
20-5925-42Epoch EclipseTM Quencher CPG 4x0.2µm €190.80 Quantity Add to Order

Description

Epoch EclipseTM Quencher CPG

Structure

Catalog Number: 20-5925-xx

Description: Epoch Eclipse™Quencher CPG

4-N-methyl-N-(4'-nitro-2'-chloroazobenzen-4-yl)-aminobutanamido-1-
(2-O-dimethoxytrityloxymethyl)-pyrrolidin-4-yl-succinoyl long chain alkylamino-CPG


F.W.: 537.89
Diluent: n/a
Coupling: This support should be used in a manner identical to normal protected nucleoside support since it contains the DMT group.
Deprotection: Deprotect using the protocol required by the nucleobases.
Storage: Freezer storage, -10 to -30°C, dry
Stability in Solution:
Please Note: This Product is for research purposes only, and may not be used for commercial, clinical, diagnostic or any other use. The Product is subject to proprietary rights of Epoch Biosciences, Inc. and is made and sold under license from Epoch Biosciences, Inc. There is no implied license for commercial use with respect to this Product and a license must be obtained directly from Epoch Biosciences, Inc. with respect to any proposed commercial use of this Product. "Commercial use" includes but is not limited to the sale, lease, license or other transfer of the Product or any material derived or produced from it, the sale, lease, license or other grant of rights to use the Product or any material derived or produced from it, or the use of the Product to perform services for a fee for third parties (including contract research). Eclipse, Gig Harbor Green, Redmond Red and Yakima Yellow are trademarks of Epoch Biosciences, Inc.

Please see Terms and Conditions of Use

EPOCH DYES AND QUENCHER

Glen Research’s agreement with Epoch Biosciences, Inc., a subsidiary of Nanogen, Inc., allows us to sell several of Epoch’s proprietary products designed for the synthesis of novel DNA probes. We are pleased to offer products based on Epoch’s Redmond Red™, Yakima Yellow™ and Gig Harbor Green™ fluorophores and Eclipse™ non-fluorescent quencher. Under our agreement we also supply PPG, a modified nucleoside and 5’-Aldehyde-Modifier C2 Phosphoramidite. The two fluorescent dyes, Yakima Yellow and Redmond Red, are available as phosphoramidites and supports. Yakima Yellow has an absorbance maximum at 530 nm and emission maximum at 549 nm, while Redmond Red’s absorbance and emission maxima are at 579 nm and 595 nm, respectively. Gig Harbor Green and 6-FAM are based on the same fluorescein core structure but Gig Harbor Green is 15-20% brighter than FAM.

The Eclipse quencher from Epoch solves most of the problems inherent in the synthesis of molecular beacon and FRET probes. The Eclipse molecule is highly stable and can be used safely in all common oligo deprotection schemes. The absorbance maximum for Eclipse Quencher is at 522 nm, compared to 479 nm for dabcyl. In addition, the structure of the Eclipse Quencher is substantially more electron deficient than that of dabcyl and this leads to better quenching over a wider range of dyes, especially those with emission maxima at longer wavelengths (red shifted) such as Redmond Red and Cy5. In addition, with an absorption range from 390 nm to 625 nm, the Eclipse Quencher is capable of effective performance in a wide range of colored FRET probes.

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200

Notes

FREQUENTLY ASKED TECHNICAL QUESTION

QUESTION: What are the relative extinction coefficients of 5'-Fluorescein, Hex and Tet etc.. at 260 nm and their Lambda max?

RESPONSE:Please see http://www.glenresearch.com/Technical/Extinctions.html

REFERENCE(S):
Oligonucleotide Properties Calculator; http://www.basic.northwestern.edu/biotools/oligocalc.html

QUESTION: What are the relative extinction coefficients of various dyes?

RESPONSE:Please see http://www.glenres.com/Technical/Extinctions.html#dyes

QUESTION: Does AMA or methylamine cause any degradation to fluorescein or fluorescein-type dyes such as FAM or FITC?

RESPONSE:Response: While AMA (Ammonium hydroxide/40% Methylamine 1:1 v/v) is considered compatible with fluorescein, the use of methylamine when deprotecting a Fluorescein-labeled oligo does lead to a small amount of degradation, which is characterized by a the appearance of a late-eluting peak by RP HPLC that shows no visible fluorescein absorbance. With standard deprotection conditions (AMA 10 minutes at 65 C) the amount of degradation is approximately 5%. The impurity is not detected with AMA at RT for 2 hours.

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200

Related products

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200