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Oligo Synthesis

Oligo Synthesis : Supports,CPGs

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Pac-dA-CPG

Pac-dA-CPG

Glen Research

Catalogue No.DescriptionPack SizePriceQty
20-2601-01Pac-dA-CPG 0.1g £14.40£13.25Offer until : 31-Dec-2019Offer Code : GLEN88% off all Glen Research products View Offer Quantity Add to Order
20-2601-02Pac-dA-CPG 0.25g £32.00£29.44Offer until : 31-Dec-2019Offer Code : GLEN88% off all Glen Research products View Offer Quantity Add to Order
20-2601-10Pac-dA-CPG 1.0g £120.00£110.40Offer until : 31-Dec-2019Offer Code : GLEN88% off all Glen Research products View Offer Quantity Add to Order
20-2701-13Pac-dA-CPG 1x10µm £80.00£73.60Offer until : 31-Dec-2019Offer Code : GLEN88% off all Glen Research products View Offer Quantity Add to Order
20-2701-41Pac-dA-CPG 4x1.0µm £48.00£44.16Offer until : 31-Dec-2019Offer Code : GLEN88% off all Glen Research products View Offer Quantity Add to Order
20-2701-42Pac-dA-CPG 4x0.2µm £32.00£29.44Offer until : 31-Dec-2019Offer Code : GLEN88% off all Glen Research products View Offer Quantity Add to Order
20-2701-45Pac-dA-CPG 4x40nm £32.00£29.44Offer until : 31-Dec-2019Offer Code : GLEN88% off all Glen Research products View Offer Quantity Add to Order
20-2801-14Pac-dA-CPG 1x15µm £120.00£110.40Offer until : 31-Dec-2019Offer Code : GLEN88% off all Glen Research products View Offer Quantity Add to Order
20-2801-41Pac-dA-CPG 4x1.0µm £48.00£44.16Offer until : 31-Dec-2019Offer Code : GLEN88% off all Glen Research products View Offer Quantity Add to Order
20-2801-42Pac-dA-CPG 4x0.2µm £32.00£29.44Offer until : 31-Dec-2019Offer Code : GLEN88% off all Glen Research products View Offer Quantity Add to Order
20-2801-45Pac-dA-CPG 4x40nm £32.00£29.44Offer until : 31-Dec-2019Offer Code : GLEN88% off all Glen Research products View Offer Quantity Add to Order

Pac-dA-CPG

Pac-dA-CPG

Glen Research

Pac-dA-CPG

Structure

 

Catalog Number: 20-2601-xx

(20-2701-xx, 20-2801-xx)

Description: Pac-dA-CPG

5'-Dimethoxytrityl-N-phenoxyacetyl-2'-deoxyAdenosine,
3'-succinoyl-long chain alkylamino-CPG


F.W.: 313.21

Diluent: Not Applicable
Coupling: This support should be used in a manner identical to normal protected nucleoside support since it contains the DMT group.
Deprotection: ultraMILD deprotection: 0.05M Potassium Carbonate in Methanol, 4 hours at Room Temperature OR 2 hours in Ammonium Hydroxide at room temperature.
Storage: Refrigerated storage, maximum of 2-8°C, dry
Stability in Solution: Not Applicable

ULTRAMILD CE PHOSPHORAMIDITES

The synthesis of labelled oligonucleotides has become a standard procedure in many laboratories and many labelling reagents, e.g., biotin and fluorescein, are now available as ß-cyanoethyl (CE) phosphoramidites. Labels which are currently available as CE phosphoramidites have one common property - they must be stable to the strongly alkaline conditions required for removal of the base protecting groups. This property is lacking in several interesting dyes and labels. We sought an alternative protecting scheme for the normal CE phosphoramidites which would allow UltraMILD deprotection and would not react with a wider variety of tags and labels. A set of monomers using phenoxyacetyl (Pac) protected dA and 4-isopropyl-phenoxyacetyl (iPr-Pac) protected dG, along with acetyl protected dC, met the desired criteria for UltraMILD deprotection.

We recommend the use of phenoxyacetic anhydride (Pac2O) in Cap A. This modification removes the possibility of exchange of the iPr-Pac protecting group on the dG with acetate from the acetic anhydride capping mix. Cleavage and deprotection can be carried out in 2 hours at room temperature with ammonium hydroxide or 4 hours with 0.05M potassium carbonate in methanol.

Isobutyryl (iBu) protected dA has been discontinued. Pac-dA is the replacement.

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

Pac-dA-CPG

Pac-dA-CPG

Glen Research

Material Safety Data Sheet

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200