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Oligo Synthesis

Oligo Synthesis : CEPs

Prices quoted are for single packs only. For multiples of the same product please request a quote. Some of Glen's products are hazardous and may be subject to additional shipping charges. Full product information is available on Glen Research's website.

Perylene-dU-CE Phosphoramidite

Perylene-dU-CE Phosphoramidite

Glen Research

Catalogue No.DescriptionPack SizePriceQty
10-1591-02Perylene-dU-CE Phosphoramidite 0.25g £600.00 Quantity Add to Order
10-1591-90Perylene-dU-CE Phosphoramidite 100µmoles £256.00 Quantity Add to Order
10-1591-95Perylene-dU-CE Phosphoramidite 50µmoles £136.00 Quantity Add to Order

Perylene-dU-CE Phosphoramidite

Perylene-dU-CE Phosphoramidite

Glen Research

Perylene-dU-CE Phosphoramidite

Structure

Catalog Number: 10-1591-xx

Description: Perylene-dU-CE Phosphoramidite

5'-Dimethoxytrityl-5-(perylen-3-yl-ethynyl)-2'-deoxyUridine,
3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
Formula: C61H57N4O8P M.W.: 1005.10 F.W.: 564.48

Diluent: Anhydrous Acetonitrile/Dichloromethane 1:1 (v/v)
Coupling: A 6 minute coupling time is recommended.
Deprotection: No changes needed from standard method recommended by synthesizer manufacturer.
Storage: Refrigerated storage, maximum of 2-8°C, dry
Stability in Solution: 1-2days

When a benzylcarbamoyl analogue of AP-dC (G-Clamp) was synthesized, it was found that, when incorporated into an oligo, it exhibited similar fluorescence to AP-dC. However, when base-paired against the 8-oxo-dG, its fluorescence was severely quenched. Rather remarkably, however, when base paired with dG or any of the other bases, A, C or T, there was no change in fluorescence – making it a specific probe for 8-oxo-dG.

By attaching pyrene or perylene to the 5 position of deoxyuridine through a triple bond, the fluorophore is electronically coupled to the deoxyuridine base. This electronic coupling of the base and the fluorophore makes the fluorescence sensitive to the base pairing of the dU portion of the molecule, allowing the discrimination between perfect and one base mismatched targets.

The base analogue tCnitro is a FRET-acceptor together with tCO (or tC) as the donor molecule. This constitutes the first ever description of a nucleobase FRET-pair. This novel FRET-pair provides a unique tool for investigations of nucleic acid containing systems. tCnitro is virtually non-fluorescent under normal conditions.

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Perylene-dU-CE Phosphoramidite

Perylene-dU-CE Phosphoramidite

Glen Research

Material Safety Data Sheet

Glen Report 19.2: Versatile New Reagents ? Pyrene-dU and Perylene-dU

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Perylene-dU-CE Phosphoramidite

Perylene-dU-CE Phosphoramidite

Glen Research

DILUTION/COUPLING DATA

The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.

ABI 392/394
Cat.No. Pack
Size
Grams/
Pack
0.1M Dil.
(mL)
LV40 LV200 40nm 0.2µm 1µm 10µm
Approximate Number of Additions
10-1591-95 50µmoles .05grams .5 3.33 2 1.25 .91 .67 .17
10-1591-90 100µmoles .101grams 1 20 12 7.5 5.45 4 1
10-1591-02 0.25grams .25grams 2.49 69.67 41.8 26.13 19 13.93 3.48
Expedite
Cat.No. Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity 50nm 0.2µm 1µm 15µm
Approximate Number of Additions
10-1591-95 50µmoles .05grams .75 .07 8.6 5.38 3.91 .54
10-1591-90 100µmoles .101grams 1.5 .07 23.6 14.75 10.73 1.48
10-1591-02 0.25grams .25grams 3.71 .07 67.8 42.38 30.82 4.24
Beckman
Cat.No. Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity 30nm 200nm 1000nm

Approximate Number of Additions
10-1591-95 50µmoles .05grams .75 .07 10.2 6.38 4.64

10-1591-90 100µmoles .101grams 1.5 .07 25.2 15.75 11.45

10-1591-02 0.25grams .25grams 3.71 .07 69.4 43.38 31.55

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