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Oligo Synthesis

Oligo Synthesis : CEPs

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For multiples of the same product please request a quote.

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Spacer Phosphoramidite C3

Spacer Phosphoramidite C3

Glen Research

Catalogue No.DescriptionPack SizePriceQty
10-1913-02Spacer Phosphoramidite C3 0.25g £192.00£163.20Offer until : 30-Sep-2019Offer Code : GLEN1515% offer orders over £1000 View Offer Quantity Add to Order
10-1913-90Spacer Phosphoramidite C3 100µmoles £60.00£51.00Offer until : 30-Sep-2019Offer Code : GLEN1515% offer orders over £1000 View Offer Quantity Add to Order

Spacer Phosphoramidite C3

Spacer Phosphoramidite C3

Glen Research

Spacer Phosphoramidite C3

Structure

Catalog Number: 10-1913-xx

Description: Spacer Phosphoramidite C3

3-(4,4'-Dimethoxytrityloxy)propyl-1-[(2-cyanoethyl)-(N,N-diisopropyl)]-
phosphoramidite
Formula: C33H43N2O5P M.W.: 578.69 F.W.: 138.06

Diluent: Anhydrous Acetonitrile
Add fresh diluent to product vial to recommended concentration and swirl vial occasionally over several minutes until product is completely dissolved. (Some oils may require between 5 and 10 minutes.) Use care to maintain anhydrous conditions. In case of transfer to alternate vial type, ensure recipient vial has been pre-dried. For more information, see http://www.glenres.com/Technical/TB_ABITransfer.pdf.
Coupling: No changes needed from standard method recommended by synthesizer manufacturer.
Deprotection: No changes needed from standard method recommended by synthesizer manufacturer.
Storage: Freezer storage, -10 to -30°C, dry
Stability in Solution: 2-3 days

SPACER MODIFIERS

The spacer phosphoramidites C3, 9, C12 and 18 are used to insert a spacer arm in an oligonucleotide. The compounds may be added in multiple additions when a longer spacer is required. 3’-Spacer C3 CPG may also act as a blocker of exonuclease and polymerase activity at the 3’-terminus. dSpacer is used to introduce a stable abasic site within an oligonucleotide. PC Spacer is a photocleavable C3 spacer modifier, part of our line of photocleavable (PC) modifiers

Item

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

Spacer Phosphoramidite C3

Spacer Phosphoramidite C3

Glen Research

Material Safety Data Sheet

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

Spacer Phosphoramidite C3

Spacer Phosphoramidite C3

Glen Research

DILUTION/COUPLING DATA

The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.

ABI 392/394
Cat.No. Pack
Size
Grams/
Pack
0.1M Dil.
(mL)
LV40 LV200 40nm 0.2µm 1µm 10µm
Approximate Number of Additions
10-1913-02 0.25grams .25grams 4.32 130.67 78.4 49 35.64 26.13 6.53
10-1913-90 100µmoles .058grams 1 20 12 7.5 5.45 4 1
Expedite
Cat.No. Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity 50nm 0.2µm 1µm 15µm
Approximate Number of Additions
10-1913-02 0.25grams .25grams 6.45 .07 122.6 76.63 55.73 7.66
10-1913-90 100µmoles .058grams 1.5 .07 23.6 14.75 10.73 1.48
Beckman
Cat.No. Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity 30nm 200nm 1000nm

Approximate Number of Additions
10-1913-02 0.25grams .25grams 6.45 .07 124.2 77.63 56.45

10-1913-90 100µmoles .058grams 1.5 .07 25.2 15.75 11.45

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200