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Oligo Synthesis

Oligo Synthesis : CEPs

Prices quoted are for single packs only. For multiples of the same product please request a quote. Some of Glen's products are hazardous and may be subject to additional shipping charges. Full product information is available on Glen Research's website.

U-CE Phosphoramidite

U-CE Phosphoramidite

Glen Research

Catalogue No.DescriptionPack SizePriceQty
10-3030-02U-CE Phosphoramidite 0.25g £32.00 Quantity Add to Order
10-3030-05U-CE Phosphoramidite 0.5g £64.00 Quantity Add to Order
10-3030-10U-CE Phosphoramidite 1.0g £128.00 Quantity Add to Order

U-CE Phosphoramidite

U-CE Phosphoramidite

Glen Research

U-CE Phosphoramidite

Catalog Number: 10-3030-xx

Description: U-CE Phosphoramidite

5'-Dimethoxytrityl-Uridine,2'-O-TBDMS,3'-[(2-cyanoethyl)-
(N,N-diisopropyl)]-phosphoramidite
Formula: C45H61N4O9PSi M.W.: 861.06 F.W.: 306.17

Diluent: Anhydrous Acetonitrile
Coupling: 12 minute coupling time recommended
Deprotection: RNA Deprotection-see bulletin Technical Bulletin
Storage: Freezer storage, -10 to -30°C, dry
Stability in Solution: 24 hours

Structure

TOM-PROTECTED RNA PHOSPHORAMIDITES

RNA synthesis using monomers containing the 2’-O-TriisopropylsilylOxyMethyl (TOM) group (TOM-Protecting-Group™) is characterized by very high coupling efficiency along with fast, simple deprotection. High coupling efficiency is achieved because the TOM-Protecting-Group exhibits lower steric hindrance than the 2’-O-t-butyldimethylsilyl (TBDMS) group used in our alternative RNA monomers. Fast and reliable deprotection is achieved using methylamine in ethanol/water at room temperature. A further feature of the TOM-Protecting-Group is that during basic steps it can not undergo 2’ to 3’ migration. This migration under basic conditions leads to non-biologically active 2’-5’ linkages when using the TBDMS group. These features allow the TOM-Protected monomers to produce longer oligonucleotides. TOM-Protected RNA monomers are also fully compatible with minor bases with 2’-O-TBDMS protection.

TBDMS-PROTECTED RNA PHOSPHORAMIDITES

Glen Research CE (ß-cyanoethyl) Phosphoramidites for RNA synthesis are produced and packaged to ensure the highest performance on commercial synthesizers. Every batch is accompanied by a Certificate of Analysis and an HPLC trace, showing the results of our QC testing. RNA Phosphoramidites are synthesis-tested with a minimum coupling efficiency of 97%. Glen Research RNA monomers are packaged in industry standard vials which are specially cleaned to eliminate particulate contamination. These monomers are available in a variety of packs, including high throughput (HT) and low cost (LC). An UltraMild set is also available for situations where sensitive bases are in use. Dmf-G (10-3029) has been discontinued and may be substituted with Ac-G (10-3025).

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

U-CE Phosphoramidite

U-CE Phosphoramidite

Glen Research

U-CE Phosphoramidite

U-CE Phosphoramidite

Glen Research

DILUTION/COUPLING DATA

The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.

ABI 392/394
Cat.No. Pack
Size
Grams/
Pack
0.1M Dil.
(mL)
LV40 LV200 40nm 0.2µm 1µm 10µm
Approximate Number of Additions
10-3030-02 0.25grams .25grams 2.9 83.33 50 31.25 22.73 16.67 4.17
10-3030-05 0.5grams .5grams 5.81 180.33 108.2 67.63 49.18 36.07 9.02
10-3030-10 1.0gram 1grams 11.61 373.67 224.2 140.13 101.91 74.73 18.68
Expedite
Cat.No. Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity 50nm 0.2µm 1µm 15µm
Approximate Number of Additions
10-3030-02 0.25grams .25grams 4.33 .07 N/A N/A 16.71 N/A
10-3030-05 0.5grams .5grams 8.67 .07 N/A N/A 34.79 N/A
10-3030-10 1.0gram 1grams 17.33 .07 N/A N/A 70.88 N/A
Beckman
Cat.No. Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity 30nm 200nm 1000nm

Approximate Number of Additions
10-3030-02 0.25grams .25grams 4.33 .07 N/A 13.19 12.39

10-3030-05 0.5grams .5grams 8.67 .07 N/A 27.19 25.55

10-3030-10 1.0gram 1grams 17.33 .07 N/A 55.13 51.79

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200