Catalog Number: 10-3030-xx
Description: U-CE Phosphoramidite
Diluent: Anhydrous Acetonitrile
|Coupling: 12 minute coupling time recommended
|Deprotection: RNA Deprotection-see bulletin Technical Bulletin
|Storage: Freezer storage, -10 to -30°C, dry
|Stability in Solution: 24 hours
TOM-PROTECTED RNA PHOSPHORAMIDITES
RNA synthesis using monomers containing the 2’-O-TriisopropylsilylOxyMethyl (TOM) group (TOM-Protecting-Group™) is characterized by very high coupling efficiency along with fast, simple deprotection. High coupling efficiency is achieved because the TOM-Protecting-Group exhibits lower steric hindrance than the 2’-O-t-butyldimethylsilyl (TBDMS) group used in our alternative RNA monomers. Fast and reliable deprotection is achieved using methylamine in ethanol/water at room temperature. A further feature of the TOM-Protecting-Group is that during basic steps it can not undergo 2’ to 3’ migration. This migration under basic conditions leads to non-biologically active 2’-5’ linkages when using the TBDMS group. These features allow the TOM-Protected monomers to produce longer oligonucleotides. TOM-Protected RNA monomers are also fully compatible with minor bases with 2’-O-TBDMS protection.
TBDMS-PROTECTED RNA PHOSPHORAMIDITES
Glen Research CE (ß-cyanoethyl) Phosphoramidites for RNA synthesis are produced and packaged to ensure the highest performance on commercial synthesizers. Every batch is accompanied by a Certificate of Analysis and an HPLC trace, showing the results of our QC testing. RNA Phosphoramidites are synthesis-tested with a minimum coupling efficiency of 97%. Glen Research RNA monomers are packaged in industry standard vials which are specially cleaned to eliminate particulate contamination. These monomers are available in a variety of packs, including high throughput (HT) and low cost (LC). An UltraMild set is also available for situations where sensitive bases are in use. Dmf-G (10-3029) has been discontinued and may be substituted with Ac-G (10-3025).
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