Catalog Number: 10-3034-xx
Description: U-TOM-CE Phosphoramidite
5'-Dimethoxytrityl-Uridine,2'-O-triisopropylsilyloxymethyl-
3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite |
| Formula: C49H69N4O10PSi |
M.W.: 933.17 |
F.W.: 306.17 |
Diluent: Anhydrous Acetonitrile |
| Coupling: See Technical Bulletin |
| Deprotection: See Technical Bulletin for details (Technical Bulletin). |
| Storage: Freezer storage, -10 to -30°C, dry |
| Stability in Solution: 24 hours |
| Please Note: TOM-RNA Phosphoramidites are supplied under agreement with QIAGEN. RNA synthesis using the TOM-Protecting-Group is covered by US Patent No. 5,986,084. |
TOM-PROTECTED RNA PHOSPHORAMIDITES
RNA synthesis using monomers containing the 2’-O-TriisopropylsilylOxyMethyl (TOM) group (TOM-Protecting-Group™) is characterized by very high coupling efficiency along with fast, simple deprotection. High coupling efficiency is achieved because the TOM-Protecting-Group exhibits lower steric hindrance than the 2’-O-t-butyldimethylsilyl (TBDMS) group used in our alternative RNA monomers. Fast and reliable deprotection is achieved using methylamine in ethanol/water at room temperature. A further feature of the TOM-Protecting-Group is that during basic steps it can not undergo 2’ to 3’ migration. This migration under basic conditions leads to non-biologically active 2’-5’ linkages when using the TBDMS group. These features allow the TOM-Protected monomers to produce longer oligonucleotides. TOM-Protected RNA monomers are also fully compatible with minor bases with 2’-O-TBDMS protection.
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