Cambio - Excellence in Molecular Biology

Lab Reagents

Lab Reagents: Smart Buffers and Reagents

Biological buffers for the life science laboratory need to meet a wide array of criteria. These include good stability, lack of toxicity, precision in pH, pKa between 6.0 and 8.0 (the region in which most biological reactions occur), minimal salt effects due to the ionic composition of the solution, free of enzymatic and hydrolytic activity and minimal participation in biological reactions. Buffers for molecular biology applications, e.g. Tris-Borate-EDTA and Tris-Acetate-EDTA, need to be free from DNAse and RNAse activity. Medicago's SmartBuffers meet all these criteria. They are manufactured using highly purified, analytical grade chemicals and are extensively quality tested. Manufacturing is done in a controlled clean environment according to GMP procedures. A high level of automation essentially avoids operator intervention and contact with the products. SmartBuffers are supplied as exactly pre-weighed tablets packed in containers or blister packs or as exactly pre-weighed powder mixes packed in sealed aluminium foil pouches. •Pre-mixed powder buffers or tablets with pre-set pH•Analytical grade reagents •Eliminates calculation, formulation and weighing errors •Dissolve-and-go for greater convenience •Stable at room temperature for at least 3 years •Guaranteed reproducibility

Phosphate Buffered Saline (PBS) pH 7.2 & 7.4

Among biological buffers PBS is one of the most commonly used. The buffer is isotonic and non-toxic to cells and has the ability to maintain their osmolarity. Thereby the buffer is suitable for washing procedures in cell cultures and for immunoassays such as ELISA and immuno-histochemical procedures

Medicago AB

Catalogue No.DescriptionPack SizePriceQty
MC-09-2051-100Phosphate Buffered Saline (PBS) pH 7.4, 100 ml 100 tablets £26.00 Quantity Add to Order
MC-09-2052-100Phosphate Buffered Saline (PBS) pH 7.4, 200 ml 100 tablets £43.00 Quantity Add to Order
MC-09-8912-100Phosphate Buffered Saline (PBS) pH 7.4, 500 ml 100 tablets £95.00 Quantity Add to Order
MC-09-8912-12Phosphate Buffered Saline (PBS) pH 7.4, 500 ml 12 tablets £23.00 Quantity Add to Order
MC-09-8912-6Phosphate Buffered Saline (PBS) pH 7.4, 500 ml 6 tablet sample (1 per customer) £0.00 Quantity Add to Order
MC-09-9400-10Phosphate Buffered Saline (PBS) pH 7.4, 1000 ml 10 tablets / blister pack £34.00 Quantity Add to Order
MC-09-9400-100Phosphate Buffered Saline (PBS) pH 7.4, 1000 ml 100 tablets £170.00 Quantity Add to Order
MC-09-9420-10Phosphate Buffered Saline (PBS) tablets without K pH 7.4, 1000 ml 10 tablets / blister pack £34.00 Quantity Add to Order
MC-09-9420-100Phosphate Buffered Saline (PBS) tablets without K pH 7.4, 1000 ml 100 tablets £170.00 Quantity Add to Order
MC-09-9499-100Phosphate Buffered Saline (PBS) pH 7.2, 1000 ml 100 tablets £170.00 Quantity Add to Order
MC-09-9500-100Phosphate Buffered Saline (PBS) without Potassium 100 tablets £197.00 Quantity Add to Order
MC-12-9422-1Phosphate Buffered Saline powder (PBS) pH 7.4, 25 L 1 pouch £37.00 Quantity Add to Order
MC-12-9423-5Phosphate Buffered Saline (PBS) 10x pH 7.4, 1000 ml 5 pouches £45.00 Quantity Add to Order
MC-12-9424-1Phosphate Buffered Saline powder (PBS) pH 7.4, 10 L 1 pouch £17.00 Quantity Add to Order
MC-12-9425-1Phosphate Buffered Saline powder (PBS) pH 7.4, 50 L 1 pouch £58.00 Quantity Add to Order
MC-12-9426-1Phosphate Buffered Saline powder (PBS) pH 7.4, 100 L 1 pouch £93.00 Quantity Add to Order

Phosphate Buffered Saline (PBS) pH 7.2 & 7.4

Among biological buffers PBS is one of the most commonly used. The buffer is isotonic and non-toxic to cells and has the ability to maintain their osmolarity. Thereby the buffer is suitable for washing procedures in cell cultures and for immunoassays such as ELISA and immuno-histochemical procedures

Medicago AB

Features

  • Formulated from analytical grade reagents
  • Autoclavable
  • Choice of two compositions: with or without potassium
  • Available as convenient tablets or powder mix in pouches
  • Ready to use in minutes

 Product description

Among biological buffers PBS is one of the most commonly used. The buffer is isotonic and non-toxic to cells and has the ability to maintain their osmolarity. Thereby the buffer is suitable for washing procedures in cell cultures and for immunoassays such as ELISA and immuno-histochemical procedures. It is often used for sample dilution in molecular biology and as protein diluent in Western blotting. Furthermore, the buffer can function as an equilibrator for gel filter columns (1).

Medicago’s PBS is specifically developed for immunological and microbiological laboratories. It is provided as pre-weighed
tablets in containers and in convenient blister packs, or as pre-weighed powder mix in sealed pouches. There are seventeen standard-sized packages and final volumes range from 100 ml to 100 litres. Choose from different pH, 7.2 and 7.4 and from PBS with or without potassium. PBS without potassium is available in two different molar strengths, 0.01 M and 0.02 M. PBS solution is supplied as a ready-to-use solution.

 

Directions for use

Empty one pouch or deposit one tablet in a laboratory flask or beaker placed on a magnetic stirrer. Add 50 ml to 500 ml of deionized water for pouches and tablets in the volume range 100 ml to 1000 ml and stir the solution for a few minutes. Adjust the water up to the given volume, stir until full dissolution and the buffer is ready to use. For larger quantities, 10 to 100 litres of buffer, use suitable equipment to stir and dissolve the buffer in compliance with the instructions above.

 Shipping and storage

PBS buffer is shipped at room temperature. Store the tablets and pouches in a dry place at room temperature. Shelf life is three years after production date.. Medicago’s ready-to-use PBS solution in bottles is stable for one year at 4°C.

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

Phosphate Buffered Saline (PBS) pH 7.2 & 7.4

Among biological buffers PBS is one of the most commonly used. The buffer is isotonic and non-toxic to cells and has the ability to maintain their osmolarity. Thereby the buffer is suitable for washing procedures in cell cultures and for immunoassays such as ELISA and immuno-histochemical procedures

Medicago AB

References

1. A peptide carrier for the delivery of biologically active proteins into mammalian cells. (2001) MC Morris, J Depollier, J Mery, F Heitz.Nature Biotechnology 19, 1173 - 1176.

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

Phosphate Buffered Saline (PBS) pH 7.2 & 7.4

Among biological buffers PBS is one of the most commonly used. The buffer is isotonic and non-toxic to cells and has the ability to maintain their osmolarity. Thereby the buffer is suitable for washing procedures in cell cultures and for immunoassays such as ELISA and immuno-histochemical procedures

Medicago AB

Applications

  • Immunoassays
  • Immuno-histochemical procedures
  • Microbiological procedures
  • Tissue and cell culture procedures
  • Sample dilution

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200