Cambio - Excellence in Molecular Biology

Lab Reagents

Lab Reagents: Absolute Lectins

Absolute Lectins, perfect for Glycoscience Medicago is a primary manufacturer of a wide variety of exceptionally pure lectins purified by affinity chromatography. Lectins are non-enzymatic proteins of non-immunoglobulin origin that bind specifically and reversibly to carbohydrate moieties without altering the covalent structure of the glycosyl ligands. The term ‘lectin’ is derived from the latin word legere, meaning ‘to select’. The specificity of a lectin is usually defined by the monosaccharides or oligosaccharides that are best at inhibiting the agglutination or precipitation caused by the lectin. Lectins are usually of plant origin but do occur in many types of organism; some are glycoproteins and may be soluble or membrane-bound. The biological function of lectins is far from fully explored. Their specificity enables binding to glycoproteins and polysaccharides as well as agglutination of erythrocytes and stimulation of blood lymphocytes. Because of their ability to distinguish glycosyl ligands on human red blood cells, lectins can be used for blood typing.Immobilized lectins can be used in affinity chromatography to purify and isolate glycoproteins, glycolipids, polysaccharides, viruses and cells. Carbohydrate-containing substances bound to the lectin may be eluted with a competitive binding substance. Medicago offers lectins as a freeze-dried powder or as a solution manufactured under aseptic conditions. If you don’t find the lectin you are looking for, we can certainly produce it for you. Furthermore, we offer different custom lectin conjugates on request. •Ultrapure quality •High activity •Wide range •Lyophilized powder •Custom lectins and conjugates

Tris Buffered Saline (TBS)

TBS is isotonic and non-toxic to cells and is suitable for molecular biology. The buffer is commonly used as substance diluent or as wash buffer in immunoassays such as ELISA. It is used in immuno-histochemical staining when the background is high and for diluting alkaline phosphatase or peroxidase-conjugated antibodies in Western blotting.

Medicago AB

Catalogue No.DescriptionPack SizePriceQty
MC-09-7500-10Tris Buffered Saline (TBS) pH 7.6, 500 ml 10 tablets £25.00 Quantity Add to Order
MC-09-7500-100Tris Buffered Saline (TBS) pH 7.6, 500 ml 100 tablets £139.00 Quantity Add to Order
MC-09-7500-5Tris Buffered Saline (TBS) pH 7.6, 500 ml 5 tablet sample (1 per customer) £0.00 Quantity Add to Order
MC-12-9133-10Tris Buffered Saline (TBS) pH 8.0, 1000 ml 10 pouches £50.00 Quantity Add to Order
MC-12-9134-10Tris Buffered Saline (TBS) 10x pH 8.0, 1000 ml 10 pouches £353.00 Quantity Add to Order

Tris Buffered Saline (TBS)

TBS is isotonic and non-toxic to cells and is suitable for molecular biology. The buffer is commonly used as substance diluent or as wash buffer in immunoassays such as ELISA. It is used in immuno-histochemical staining when the background is high and for diluting alkaline phosphatase or peroxidase-conjugated antibodies in Western blotting.

Medicago AB

Product description

TBS is isotonic and non-toxic to cells and is suitable for molecular biology. The buffer is commonly used as substance diluent or as wash buffer in immunoassays such as ELISA. It is used in immuno-histochemical staining when the background is high and for diluting alkaline phosphatase or peroxidase-conjugated antibodies in Western blotting.

Medicago’s TBS buffer is supplied as pre-weighed tablets in
bottles and in blister packs or as powder in sealed pouches. One tablet or the contents of one pouch gives 500 ml or 1000 ml of buffer solution in the compositions:

1. 1 x solution; 0.05 M Tris buffered saline, 0.138 M sodium chloride, 0.0027 M potassium chloride with pH 8.0 at 25°C.

2. 10 x solution; 0.5 M Tris buffered saline, 1.38 M sodium
chloride, 0.027 M potassium chloride with pH 8.0 at 25°C.

3. 1 x solution; 0.05 M Tris-HCl buffer, 0.15 M sodium chloride with pH 7.6 at 25°C.

Features

  • Formulated from analytical grade reagents
  • Isotonic, non-toxic buffer
  • Choice of 1x or 10x concentration
  • Guaranteed reproducibility
  • Ready to use in minutes

 

Directions for use

Empty one pouch or dispose one tablet in a laboratory flask or beaker placed on a magnetic stirrer. Add 200 ml of deionized water for the tablets and 500 ml for the pouches and stir the solution for a few minutes. Adjust the volume up to 500 ml or 1000 ml, stir until full dissolution and the buffer is ready to use.

Shipping and storage

TBS buffer is shipped at room temperature. Store tablets and pouches in a dry place at room temperature. Shelf life is three years after production date.

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

Tris Buffered Saline (TBS)

TBS is isotonic and non-toxic to cells and is suitable for molecular biology. The buffer is commonly used as substance diluent or as wash buffer in immunoassays such as ELISA. It is used in immuno-histochemical staining when the background is high and for diluting alkaline phosphatase or peroxidase-conjugated antibodies in Western blotting.

Medicago AB

Applications

  • Wash buffer in immunoassays
  • Anibody diluent in Western blotting
  • Sample diluent in in vitro diagnostics
  • Immuno-histochemical staining
  • In Situ hybridization

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200