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Lab Reagents

Lab Reagents: Absolute Lectins

Absolute Lectins, perfect for Glycoscience Medicago is a primary manufacturer of a wide variety of exceptionally pure lectins purified by affinity chromatography. Lectins are non-enzymatic proteins of non-immunoglobulin origin that bind specifically and reversibly to carbohydrate moieties without altering the covalent structure of the glycosyl ligands. The term ‘lectin’ is derived from the latin word legere, meaning ‘to select’. The specificity of a lectin is usually defined by the monosaccharides or oligosaccharides that are best at inhibiting the agglutination or precipitation caused by the lectin. Lectins are usually of plant origin but do occur in many types of organism; some are glycoproteins and may be soluble or membrane-bound. The biological function of lectins is far from fully explored. Their specificity enables binding to glycoproteins and polysaccharides as well as agglutination of erythrocytes and stimulation of blood lymphocytes. Because of their ability to distinguish glycosyl ligands on human red blood cells, lectins can be used for blood typing.Immobilized lectins can be used in affinity chromatography to purify and isolate glycoproteins, glycolipids, polysaccharides, viruses and cells. Carbohydrate-containing substances bound to the lectin may be eluted with a competitive binding substance. Medicago offers lectins as a freeze-dried powder or as a solution manufactured under aseptic conditions. If you don’t find the lectin you are looking for, we can certainly produce it for you. Furthermore, we offer different custom lectin conjugates on request. •Ultrapure quality •High activity •Wide range •Lyophilized powder •Custom lectins and conjugates

Crotalaria juncea lectin

rotalaria juncea lectin is isolated from Sunn hemp seeds and purified by affinity chromatography. The lectin has a molecular weight of 124 kDa and is composed of four identical polypeptide chains of 31 kDa. The lectin has non-specific blood group activity (1). It displays specificity toward ß-galactosides and interacts with serum glycoproteins, cytochrome b5 and virus surface glycoproteins such as BVDV, influenza and bovine diarrhea

Medicago AB

Catalogue No.DescriptionPack SizePriceQty
MC-05-0105-10Crotalaria juncea lectin 10 mg £80.00 Quantity Add to Order
MC-05-0105-50Crotalaria juncea lectin 50 £266.00 Quantity Add to Order

Crotalaria juncea lectin

rotalaria juncea lectin is isolated from Sunn hemp seeds and purified by affinity chromatography. The lectin has a molecular weight of 124 kDa and is composed of four identical polypeptide chains of 31 kDa. The lectin has non-specific blood group activity (1). It displays specificity toward ß-galactosides and interacts with serum glycoproteins, cytochrome b5 and virus surface glycoproteins such as BVDV, influenza and bovine diarrhea

Medicago AB

Product description

Crotalaria juncea lectin is isolated from Sunn hemp seeds and purified by affinity chromatography. The lectin has a molecular weight of 124 kDa and is composed of four identical polypeptide chains of 31 kDa. The lectin has non-specific blood group activity (1). It displays specificity toward ß-galactosides and interacts with serum glycoproteins, cytochrome b5 and virus surface glycoproteins such as BVDV, influenza and bovine diarrhea (1).

Medicago’s Crotalaria juncea is supplied as a white to yellowish lyophilized powder. The purity of Crotalaria juncea lectin is determined by SDS- PAGE, which generates one single band at 31 kDa corresponding to the four identical polypeptide chains. The lectin is available in vials containing 50 mg or 10 mg powder. The product is to be used for laboratory work only.

Features 

  • Ultrapure quality
  • Non-specific blood group activity
  • Sugar specificity: Gal > GalNAc
  • Phytohaemagglutinin
  • Lyophilized powder


Directions for use

The lectin may be reconstituted with 2 ml of deionized water before use. Spin the vial gently until full dissolution. Aggregation is thought to occur in the presence of high concentrations of 2-mercaptoethanol. The solution may be reconstituted in this buffer to desired working concentration. In absence of lactose the lectin will polymerize and storage at pH 8.6–8.8 causes precipitation.

 Shipping and storage

The product is shipped at -20°C however for over-the-day transport it may be shipped at ambient temperature. The lyophilized powder is stable for more than three years from production date when stored below -20°C. After reconstitution with deionized water, the solution may be stored frozen in working aliquots for up to 12 months.

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

Crotalaria juncea lectin

rotalaria juncea lectin is isolated from Sunn hemp seeds and purified by affinity chromatography. The lectin has a molecular weight of 124 kDa and is composed of four identical polypeptide chains of 31 kDa. The lectin has non-specific blood group activity (1). It displays specificity toward ß-galactosides and interacts with serum glycoproteins, cytochrome b5 and virus surface glycoproteins such as BVDV, influenza and bovine diarrhea

Medicago AB

References

(1) Liener I. E., Sharon N., Goldstein I. J., (1986) The Lectins – Properties, Functions and Applications in Biology and Medicine.

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

Crotalaria juncea lectin

rotalaria juncea lectin is isolated from Sunn hemp seeds and purified by affinity chromatography. The lectin has a molecular weight of 124 kDa and is composed of four identical polypeptide chains of 31 kDa. The lectin has non-specific blood group activity (1). It displays specificity toward ß-galactosides and interacts with serum glycoproteins, cytochrome b5 and virus surface glycoproteins such as BVDV, influenza and bovine diarrhea

Medicago AB

Specifications

Appearance: White to yellowish lyophilized powder                              

Source: Sunn hemp seeds

Molecular weight: 124 kDa

Sugar specificity: Gal > GalNAc

Activity: Blood group non-specific

Microorganisms: < 100 CFU/g

Protein content: > 90 %, OD280nm (ε 1mg/ml = 1.14), > 95%, essentially salt free

Identity: SDS-PAGE, one band at 31 kDa

Shelf life: > Three years when stored at -20

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

Crotalaria juncea lectin

rotalaria juncea lectin is isolated from Sunn hemp seeds and purified by affinity chromatography. The lectin has a molecular weight of 124 kDa and is composed of four identical polypeptide chains of 31 kDa. The lectin has non-specific blood group activity (1). It displays specificity toward ß-galactosides and interacts with serum glycoproteins, cytochrome b5 and virus surface glycoproteins such as BVDV, influenza and bovine diarrhea

Medicago AB

Applications

  • Studies of virus surface glycoproteins
  • Purifying bovine diarrhea virus when immobilized an agarose gel

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200