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Lab Reagents

Lab Reagents: Absolute Lectins

Absolute Lectins, perfect for Glycoscience Medicago is a primary manufacturer of a wide variety of exceptionally pure lectins purified by affinity chromatography. Lectins are non-enzymatic proteins of non-immunoglobulin origin that bind specifically and reversibly to carbohydrate moieties without altering the covalent structure of the glycosyl ligands. The term ‘lectin’ is derived from the latin word legere, meaning ‘to select’. The specificity of a lectin is usually defined by the monosaccharides or oligosaccharides that are best at inhibiting the agglutination or precipitation caused by the lectin. Lectins are usually of plant origin but do occur in many types of organism; some are glycoproteins and may be soluble or membrane-bound. The biological function of lectins is far from fully explored. Their specificity enables binding to glycoproteins and polysaccharides as well as agglutination of erythrocytes and stimulation of blood lymphocytes. Because of their ability to distinguish glycosyl ligands on human red blood cells, lectins can be used for blood typing.Immobilized lectins can be used in affinity chromatography to purify and isolate glycoproteins, glycolipids, polysaccharides, viruses and cells. Carbohydrate-containing substances bound to the lectin may be eluted with a competitive binding substance. Medicago offers lectins as a freeze-dried powder or as a solution manufactured under aseptic conditions. If you don’t find the lectin you are looking for, we can certainly produce it for you. Furthermore, we offer different custom lectin conjugates on request. •Ultrapure quality •High activity •Wide range •Lyophilized powder •Custom lectins and conjugates

Lens culinaris lectin (LCA/LcH)

Lens culinaris lectin or agglutinin (LCA) is isolated from Lens culinaris (lentil) seeds and purified by affinity chromatography. The lectin has two subunits and a molecular weight of 46 kDa (1) and it forms a complex together with sucrose.

Medicago AB

Catalogue No.DescriptionPack SizePriceQty
MC-05-0104-10Lens culinaris lectin (LCA/LcH)10 mg £61.00 Quantity Add to Order
MC-05-0104-100Lens culinaris lectin (LCA/LcH)100 mg £435.00 Quantity Add to Order
MC-05-0104-25Lens culinaris lectin (LCA/LcH)25 mg £109.00 Quantity Add to Order

Lens culinaris lectin (LCA/LcH)

Lens culinaris lectin or agglutinin (LCA) is isolated from Lens culinaris (lentil) seeds and purified by affinity chromatography. The lectin has two subunits and a molecular weight of 46 kDa (1) and it forms a complex together with sucrose.

Medicago AB

Product description

Lens culinaris lectin or agglutinin (LCA) is isolated from Lens culinaris (lentil) seeds and purified by affinity chromatography. The lectin has two subunits and a molecular weight of 46 kDa (1) and it forms a complex together with sucrose.

Lectins are, due to their specific binding to carbohydrate struc- tures on the cell surface or elsewhere useful in haematology, immunology or as specific markers for membrane glycoprotein structures (2). LCA’s carbohydrate specificity is D-mannose and D-glucose and the two isomers LCA-A and LCA-B agglutinates human red blood cells, although this reaction is not blood group specific. LCA is also a useful component in affinity chroma- tography columns for the separation of glycoconjugates (3).

Medicago’s Lens culinaris lectin is supplied as a white lyophilized powder from 1 mM CaCl2, 1 mM MnCI2 and 1 mM MgCI2. No preservatives are added. In isoelectric focusing, the lectin generates two major bands at pI 8.15, 8.45 and one minor band at pI 8.65 (Figure 3).The activity of the lectin is determined by haemagglutination with human blood. Lens culinaris lectin agglutinates a 2% suspension of human erythrocytes at a lectin concentration of ≤8 μg/ml in 0.9% saline solution (NaCl) after 2 h at 25°C.

Adding 60 mM methylmannoside yields an inhibition with a lectin titer that is at least 16-fold weaker than the control (Figure 4). Addition of Mn2 and Ca2 to the reconstitution buffer will enhance hemagglutination activity (1).

The the lectin is avaliable in vials containing 100 mg, 25 mg or 10 mg lyophilized powder and the product is to be used for laboratory work only.

Directions for use

Medicago’s Lens culinaris lyophilized lectin may be reconstituted with 2 ml of deionized water before use, spin the vial gently until full dissolution. Aggregation is thought to occur in the presence of high concentrations of 2-mercaptoethanol.

 

Shipping and storage

The product is shipped at -20°C however for over-the-day transport it may be shipped at ambient temperature. The lyophilized powder is stable for more than three years from production date when stored below -20°C. After reconstitution with deionized water, the solution may be stored frozen in working aliquots for up to 12 months.


Features

  • Ultrapure quality
  • Sugar specificity: α-D-mannose and α-D-glucose
  • Haemagglutinating activity
  • Lyophilized powder

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

Lens culinaris lectin (LCA/LcH)

Lens culinaris lectin or agglutinin (LCA) is isolated from Lens culinaris (lentil) seeds and purified by affinity chromatography. The lectin has two subunits and a molecular weight of 46 kDa (1) and it forms a complex together with sucrose.

Medicago AB

References

(1) Liener I. E., Sharon N., Goldstein I. J., (1986) The Lectins – Properties, Functions and Applications in Biology and Medicine.

(2) The Structure of the Lentil (Lens culinaris) Lectin Amino acid sequence determination and prediction of the secondary structure. Andre Foriers S.,Evelyne Lebrung, Roland Varnapenbuschg,Roeland de Neve, and A. Donny Strosberggy. J. Biol. Chem. 286, No 11: 5550-5560.

(3) NMR, molecular modeling, and crystallographic studies of lentil lectin- sucrose interaction. Casset, F., Hamelryck, T., Loris, R

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

Lens culinaris lectin (LCA/LcH)

Lens culinaris lectin or agglutinin (LCA) is isolated from Lens culinaris (lentil) seeds and purified by affinity chromatography. The lectin has two subunits and a molecular weight of 46 kDa (1) and it forms a complex together with sucrose.

Medicago AB

Specifications

Appearence: White lyophilized powder or flocculate

Source: Lentil seeds

Molecular weight: 46 kDa

Sugar specificity: α-D-mannose and α-D-glucose

Activity: Agglutinates human erythrocytes in a 2 % blood suspension with lectin concentration ≤ 8 μg/ml in 0.9% NaCl after 2 h at 25°C.

Inhibition: Occurs with 60 mM metylmannoside at a minimum of 16-fold lower titer of lectin than the positive control, 1 mg/ml.

Microorganisms: < 100 CFU/g 

Protein content: >85 % protein by OD280nm (α 1mg/ml = 1.34), essentially salt free.

Identity & Purity: Isoelectric focusing, Phast gel IEF. Two major bands at pI 8.15, 8.45 and one minor at pI 8.65.

Shelf life: > Three years when stored at -20°C

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

Lens culinaris lectin (LCA/LcH)

Lens culinaris lectin or agglutinin (LCA) is isolated from Lens culinaris (lentil) seeds and purified by affinity chromatography. The lectin has two subunits and a molecular weight of 46 kDa (1) and it forms a complex together with sucrose.

Medicago AB

Applications

  • Haemagglutination studies
  • Cell agglutination studies
  • Component in affinity columns
  • Separation of glycoconjugates

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200