Cambio - Excellence in Molecular Biology

Oligo Synthesis

Oligo Synthesis : Supports,CPGs

Prices quoted are for single packs only.

For multiples of the same product please request a quote.

Some of Glen's products are hazardous and may be subject to additional shipping charges.

Full product information is available on Glen Research's web site.

3’- Amino-dT CPG

3’- Amino-dT CPG

Glen Research

Catalogue No.DescriptionPack SizePriceQty
20-2981-013’-Amino-dT CPG0.1g £96.00£88.32Offer until : 31-Dec-2019Offer Code : GLEN88% off all Glen Research products View Offer Quantity Add to Order
20-2981-103’-Amino-dT CPG1.0g £796.00£732.32Offer until : 31-Dec-2019Offer Code : GLEN88% off all Glen Research products View Offer Quantity Add to Order
20-2981-133’-Amino-dT CPGPack of 1 £400.00£368.00Offer until : 31-Dec-2019Offer Code : GLEN88% off all Glen Research products View Offer Quantity Add to Order
20-2981-14 3’-Amino-dT CPGPack of 1 £600.00£552.00Offer until : 31-Dec-2019Offer Code : GLEN88% off all Glen Research products View Offer Quantity Add to Order
20-2981-413’-Amino-dT CPGPack of 4 £160.00£147.20Offer until : 31-Dec-2019Offer Code : GLEN88% off all Glen Research products View Offer Quantity Add to Order
20-2981-423’-Amino-dT CPGPack of 4 £96.00£88.32Offer until : 31-Dec-2019Offer Code : GLEN88% off all Glen Research products View Offer Quantity Add to Order

3’- Amino-dT CPG

3’- Amino-dT CPG

Glen Research

3’- Amino-dT CPG

Structure

Catalog Number: 20-2981-xx

Description: 3'-Amino-dT CPG

5'-Dimethoxytrityl-2',3'-dideoxyThymidine-3'-amino-(N-hexafluoroglutaroyl)-CPG
Formula: C10H15N3O4   F.W.: 303.21

Coupling: No changes needed from standard method recommended by synthesizer manufacturer.
Deprotection: Deprotect with ammonium hydroxide for 17 hours at 55°C. Not compatible with AMA deprotection.
Storage: Refrigerated storage, maximum of 2-8°C, dry
Stability in Solution:

5’-LABELLING OF MICRORNAS

Several methods have been developed for the detection of miRNAs, however, few allow the simultaneous detection of multiple miRNAs. To overcome this analytical deficiency, the Richert group has recently developed an ingenious method to selectively detect miRNAs on microarrays without interference from endogenous pre-mRNAs, mRNAs and other RNA species. In this method, a short oligonucleotide containing 3’-amino-dT and a 5’ reporter molecule is chemically ligated to the microRNA in a one-step procedure by in situ activation of the microRNA. This is specifically achieved by taking advantage of the fact that miRNAs, unlike other RNAs, are 5’-phosphorylated. The reaction is template-directed (and thus sequence specific) and can be performed together with enzymatic 3’-extension/labeling, either in solution or on a support. The short DNA labeling strand may feature one of a variety of different labels, such as a biotin group or a fluorophore.

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

3’- Amino-dT CPG

3’- Amino-dT CPG

Glen Research

Material Safety Data Sheet

Glen Report 24.2: Technical Brief - Reagents for 5'-Labeling of microRNAs

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200