Proteinase K is a proteolytic enzyme. The enzyme uses Ca2+ ions for structural stability. Due to the high total activity of the enzyme, lysis can be carried out in the presence of EDTA. Proteinase K is therefore excellently useful for the extraction of nucleic acids from various biological samples.
The freeze-dried product shall be stored at 2 to 8 °C. The rehydrated stock solution must be stored at < -18 °C. Avoid repeated freezing and thawing. Preparation of aliquots might be required if more than 5 freezing/thawing cycles are required.
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