Cambio - Excellence in Molecular Biology

Enzymes for Molecular Biology

Enzymes for Molecular Biology: Enzymes for Molecular Biology

Uracil N-Glycosylase (UNG)

Uracil N-Glycosylase (UNG), also known as uracil-DNA glycosylase, hydrolyzes the N-glycosidic bond between the deoxyribose sugar and uracil in DNA.

BioSearch Tech (Lucigen/Epicentre)

Catalogue No.DescriptionPack SizePriceQty
UG131KUracil N-Glycosylase (UNG)1U/µl 1,000U £987.00£987.00Offer until : 31-Dec-2020Contact Cambio for special pricing on all orders over £2000 View Offer Quantity Add to Order

Uracil N-Glycosylase (UNG)

Uracil N-Glycosylase (UNG), also known as uracil-DNA glycosylase, hydrolyzes the N-glycosidic bond between the deoxyribose sugar and uracil in DNA.

BioSearch Tech (Lucigen/Epicentre)

Formally known as  HK™-UNG Thermolabile Uracil N-Glycosylase and the previous numbers were HU5901K and HU59100.

Uracil N-Glycosylase (UNG), also known as uracil-DNA glycosylase, hydrolyzes the N-glycosidic bond between the deoxyribose sugar and uracil in DNA containing deoxyuridine in place of thymidine. UNG is active on both ssDNA and dsDNA that contain uracil, but has no activity on RNA or 2´-deoxyuridine- 5´-monophosphate. UNG is ideal for studying repair of abasic sites in double-stranded DNA.

Unit Definition: One unit of UNG catalyzes the release of 1 nmol of uracil from uracil-containing DNA in 1 hour at 37°C under standard assay conditions.

Note: Epicentre's unit is 5- to 20-fold more active than a unit as defined by other suppliers. Therefore, Dilution Buffer is provided for applications requiring lower concentrations of enzyme.

Dilution and Storage Buffer: 50% glycerol containing 50 mM Tris-HCl (pH 7.5), 100 mM NaCl, 0.1 mM EDTA, 1 mM DTT, and 0.1% Triton® X-100.

Quality Control: UNG is free of detectable exo- and endonuclease and RNase activities.

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

Uracil N-Glycosylase (UNG)

Uracil N-Glycosylase (UNG), also known as uracil-DNA glycosylase, hydrolyzes the N-glycosidic bond between the deoxyribose sugar and uracil in DNA.

BioSearch Tech (Lucigen/Epicentre)

Protocols for: Uracil N-Glycosylase (UNG)

Uracil N-Glycosylase (UNG)

(catalogue number UG13100 or UG131K)

Please note: all protocols off site are the responsibility of the products supplier

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

Uracil N-Glycosylase (UNG)

Uracil N-Glycosylase (UNG), also known as uracil-DNA glycosylase, hydrolyzes the N-glycosidic bond between the deoxyribose sugar and uracil in DNA.

BioSearch Tech (Lucigen/Epicentre)

Note: The product name and catalog numbers have changed. The previous name was HK™-UNG Thermolabile Uracil N-Glycosylase and the previous numbers were HU5901K and HU59100.
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  2. MacFarlane, A. J., et al. (2011) Nuclear Localization of de Novo Thymidylate Biosynthesis Pathway Is Required to Prevent Uracil Accumulation in DNA. J. Biol. Chem. 286, 44015-44022
  3. Jaijo, T., et al. (2010) Microarray-Based Mutation Analysis of 183 Spanish Families with Usher Syndrome. Invest. Ophthalmol. Vis. Sci. 51, 1311-1317
  4. Tat, S. K., et al. (2010) Variable Effects of 3 Different Chondroitin Sulfate Compounds on Human Osteoarthritic Cartilage/Chondrocytes: Relevance of Purity and Production Process. J Rheumatol 37, 656-664
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  6. Tang, Y.-W. (2009) Duplex PCR Assay Simultaneously Detecting and Differentiating Bartonella quintana, B. henselae, and Coxiella burnetii in Surgical Heart Valve Specimens. J. Clin. Microbiol. 47, 2647-2650
  7. Francesconi, A., et al. (2008) Automated and Manual Methods of DNA Extraction for Aspergillus fumigatus and Rhizopus oryzae Analyzed by Quantitative Real-Time PCR. J. Clin. Microbiol. 46, 1978-1984
  8. Kasai, M., et al. (2008) Detection of a Molecular Biomarker for Zygomycetes by Quantitative PCR Assays of Plasma, Bronchoalveolar Lavage, and Lung Tissue in a Rabbit Model of Experimental Pulmonary Zygomycosis. J. Clin. Microbiol. 46, 3690-3702
  9. Francesconi, A., et al. (2006) Characterization and Comparison of Galactomannan Enzyme Immunoassay and Quantitative Real-Time PCR Assay for Detection of Aspergillus fumigatus in Bronchoalveolar Lavage Fluid from Experimental Invasive Pulmonary Aspergillosis. J. Clin. Microbiol. 44, 2475-2480
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  13. O'Sullivan, C. E., et al. (2003) Development and Validation of a Quantitative Real-Time PCR Assay Using Fluorescence Resonance Energy Transfer Technology for Detection of Aspergillus fumigatus in Experimental Invasive Pulmonary Aspergillosis. J. Clin. Microbiol. 41, 5676-5682
  14. Sloan, L. M., et al. (2002) Multiplex LightCycler PCR Assay for Detection and Differentiation of Bordetella pertussis and Bordetella parapertussis in Nasopharyngeal Specimens. J. Clin. Microbiol. 40, 96-100
  15. Smalling, T. W., et al. (2002) Molecular Approaches To Detecting Herpes Simplex Virus and Enteroviruses in the Central Nervous System. J. Clin. Microbiol. 40, 2317-2322
  16. Schwab, K. J., et al. (2001) Development of a Reverse Transcription-PCR-DNA Enzyme Immunoassay for Detection of "Norwalk-Like" Viruses and Hepatitis A Virus in Stool and Shellfish. Appl. Envir. Microbiol. 67, 742-749
  17. Atamna, H., et al. (2000) A method for detecting abasic sites in living cells: Age-dependent changes in base excision repair. PNAS 97, 686-691
  18. Pinz, K. G. and Bogenhagen, D. F. (2000) Characterization of a Catalytically Slow AP Lyase Activity in DNA Polymerase gamma and Other Family A DNA Polymerases. J. Biol. Chem. 275, 12509-12514
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If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

Uracil N-Glycosylase (UNG)

Uracil N-Glycosylase (UNG), also known as uracil-DNA glycosylase, hydrolyzes the N-glycosidic bond between the deoxyribose sugar and uracil in DNA.

BioSearch Tech (Lucigen/Epicentre)

Note: This product is accompanied by a limited non-exclusive license for the purchaser to use the purchased product for Base Excision Sequence Scanning (BESS-T&G™ Scanning) in life science research under U.S. Patent No. 6,048,696 and similar patents pending in the U.S. and other countries. No other license for any other use or field of use is either granted or implied by the sale of this product. Also see Section 6 for information on BESS-T&G™ Base Reader Kits.

Note: EPICENTRE's unit is 5- to 20-fold more active than a unit as defined by other suppliers. Therefore, Dilution Buffer is provided for applications requiring lower concentrations of enzyme

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

Uracil N-Glycosylase (UNG)

Uracil N-Glycosylase (UNG), also known as uracil-DNA glycosylase, hydrolyzes the N-glycosidic bond between the deoxyribose sugar and uracil in DNA.

BioSearch Tech (Lucigen/Epicentre)

Applications

  • DNA repair studies

 




If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200