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Enzymes for Molecular Biology

Enzymes for Molecular Biology: Enzymes for Molecular Biology

OmniCleave™ Endonuclease

OmniCleave Endonuclease is highly purified from a recombinant E. coli strain

BioSearch Tech (Lucigen/Epicentre)

Catalogue No.DescriptionPack SizePriceQty
OC7850KOmniCleave™ Endonuclease200U/µl 50,000U £222.00£222.00Offer until : 31-Dec-2020Contact Cambio for special pricing on all orders over £2000 View Offer Quantity Add to Order

OmniCleave™ Endonuclease

OmniCleave Endonuclease is highly purified from a recombinant E. coli strain

BioSearch Tech (Lucigen/Epicentre)

OmniCleave™ Endonuclease is a highly purified enzyme from a recombinant E. coli strain that degrades single- and double-stranded DNA and RNA to di-, tri-, and tetranucleotides(Figure 1). OmniCleave™ Endonuclease has the same substrate specificity and yields the same products as benzonase®, an enzyme derived from Serratia marcescens.

 

 

 

Figure 1. Removal of nucleic acids from cell lysates using OmniCleave Endonuclease. Figure 1. Removal of nucleic acids from cell lysates using OmniCleave™ Endonuclease. Cell lysates were prepared with or without OmniCleave Endonuclease from E. coli cells expressing human lactate dehydrogenase B (LDH) and E. coli alkaline phosphatase (AP). Two microliters of each of the cell lysates were separated by electrophoresis on a 1% agarose gel and the nucleic acids detected by ethidium bromide staining. Lane M, 1-kb ladder

 

 

Unit Definition:

One unit converts 1.0 OD260 (~60µg) of salmon sperm DNA into acid-soluble nucleotides in 30 minutes at 37°C in 50mM Tris-HCl, pH 8.0 at 25°C and 1mM MgCl2.

 

Storage Buffer:

50% glycerol solution containing 50mM Tris-HCl, pH 7.5 at 25°C, 0.1M NaCl, 0.1mM EDTA, 1mM dithiothreitol, and 0.1% Triton® X-100.

 

Quality Control:

OmniCleave™ Endonuclease is free of detectable protease activity.

 




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OmniCleave™ Endonuclease

OmniCleave Endonuclease is highly purified from a recombinant E. coli strain

BioSearch Tech (Lucigen/Epicentre)

Protocols for: OmniCleave™ Endonuclease

 

Due to the constant updating of the protocols by the manufacturer we have provided a direct link to Epicentre’s product page, where the latest protocol is available.

Please note this will open a new page or window on your computer.

 

OmniCleave™ Endonuclease Protocol

 

(catalogue number OC7810K / OC7850K)

Please note: all protocols off site are the responsibility of the products supplier

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

OmniCleave™ Endonuclease

OmniCleave Endonuclease is highly purified from a recombinant E. coli strain

BioSearch Tech (Lucigen/Epicentre)

References

 

  1. Molloy, MP. et al. (1998) Electrophoresis 19, 837.
  2. Herbert, BR. et al. (1998) Electrophoresis 19, 851.
  3. Chandramouli, S., et al. (2010) Serotype-Specific Structural Differences in the Protease-Cofactor Complexes of the Dengue Virus Family, J. Virol. 84 , 3059-3067.
  4. van Leeuwen, M., et al. (2010) Human Picobirnaviruses Identified by Molecular Screening of Diarrhea Samples, J. Clin. Microbiol. 48 , 1787-1794.
  5. Gilljam, K. M., et al. (2009) Identification of a novel, widespread, and functionally important PCNA-binding motif, J. Cell Biol. 186 , 645-654.
  6. Pelish, T. M. & McClain, M. S. (2009) Dominant-negative Inhibitors of the Clostridium perfringens {epsilon}-Toxin, J. Biol. Chem. 284 , 29446-29453.
  7. Otterlei, M., et al. (2006) Werner syndrome protein participates in a complex with RAD51, RAD54, RAD54B and ATR in response to ICL-induced replication arrest, J. Cell Sci. 119 , 5137-5146.
  8. Torres, V. J., et al. (2005) Functional Properties of the p33 and p55 Domains of the Helicobacter pylori Vacuolating Cytotoxin, J. Biol. Chem. 280 , 21107-21114.
  9. Fan, J., et al. (2004) XRCC1 co-localizes and physically interacts with PCNA, Nucleic Acids Res. 32 , 2193-2201.
  10. Guarino, L. A., et al. (2002) Baculovirus lef-12 Is Not Required for Viral Replication, J. Virol. 76 , 12032-12043.
  11. Sawitzke, J. A., et al. (2002) Transcriptional Interference by a Complex Formed at the Centromere-Like Partition Site of Plasmid P1, J. Bacteriol. 184 , 2447-2454.
  12. Feilmeier, B. J., et al. (2000) Green Fluorescent Protein Functions as a Reporter for Protein Localization in Escherichia coli, J. Bacteriol. 182 , 4068-4076.
  13. Feltzer, R. E., et al. (2000) Alkaline Proteinase Inhibitor of Pseudomonas aeruginosa.Interaction of native and N-terminally truncated inhibitor proteins with Pseudomonas metalloproteinases, J. Biol. Chem. 275 , 21002-21009.

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

OmniCleave™ Endonuclease

OmniCleave Endonuclease is highly purified from a recombinant E. coli strain

BioSearch Tech (Lucigen/Epicentre)

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If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200