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Enzymes for Molecular Biology

Enzymes for Molecular Biology: Enzymes for Molecular Biology

Reverse Transcriptase (HIV)

Human Immunodeficiency Virus (HIV) Reverse Transcriptase is an RNA directed DNA polymerase which can synthesize a complementary DNA strand initiating from a primer using either RNA or single-stranded DNA as a template.

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Catalogue No.DescriptionPack SizePriceQty
PM-1373-01Reverse Transcriptase (HIV)500U POA Quantity Add to Order

Reverse Transcriptase (HIV)

Human Immunodeficiency Virus (HIV) Reverse Transcriptase is an RNA directed DNA polymerase which can synthesize a complementary DNA strand initiating from a primer using either RNA or single-stranded DNA as a template.

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Description

  • Human Immunodeficiency Virus (HIV) Reverse Transcriptase is an RNA directed DNA polymerase which can synthesize a complementary DNA strand initiating from a primer using either RNA or single-stranded DNA as a template.

Technical Datasheet available here

Applications

  • Provides an excellent target for evaluating antiviral agents or inhibitors (1,2)
  • Catalyzes error-prone synthesis on DNA and RNA templates - ideal for introducing random mutations (3)

Unit Definition

One unit is the amount of enzyme required to incorporate 1 nmol of labeled dTTP into acid insoluble material in 10 minutes
at 37°C.

Concentration

10 - 30 units/µl

Assay Conditions

50 mM Tris-HCl (pH 8.3)
6 mM MgCl2
40 mM KCl
0.5 mM [3H] TTP
0.4 mM poly rA:dT
Reaction volume of 50 µl

Storage Buffer

20 mM potassium phosphate (pH 7.1)
1.0 mM dithiothreitol
0.02% Triton X-100™
50% (v/v) glycerol

Reaction Buffer

10X HIV-RT Reaction Buffer

Storage Conditions

Store at -20°C
Product shipped on Dry Ice

 

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

Reverse Transcriptase (HIV)

Human Immunodeficiency Virus (HIV) Reverse Transcriptase is an RNA directed DNA polymerase which can synthesize a complementary DNA strand initiating from a primer using either RNA or single-stranded DNA as a template.

Cambio

Click here for Data sheet

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

Reverse Transcriptase (HIV)

Human Immunodeficiency Virus (HIV) Reverse Transcriptase is an RNA directed DNA polymerase which can synthesize a complementary DNA strand initiating from a primer using either RNA or single-stranded DNA as a template.

Cambio

References

1. Hirsch, M. S., Kaplan, J. C. (1985) Ann. Intern. Med. 103, 750-755
2. Tisdale, M. et al (1989) J. Antimicrob. Chemother. 23, 47-54
3. Williams, K. J., Loeb, L. A. (1992) Curr. Top. Microbiol. Immunol. 176, 80-165

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

Reverse Transcriptase (HIV)

Human Immunodeficiency Virus (HIV) Reverse Transcriptase is an RNA directed DNA polymerase which can synthesize a complementary DNA strand initiating from a primer using either RNA or single-stranded DNA as a template.

Cambio

Applications

  • Provides an excellent target for evaluating antiviral agents or inhibitors (1,2)
  • Catalyzes error-prone synthesis on DNA and RNA templates - ideal for introducing random mutations (3)

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200