Cambio - Excellence in Molecular Biology

Gene Expression Analysis

Gene Expression Analysis: Gene Expression Analysis

MessageBooster™ cDNA Synthesis from Cell Lysates Kit

The MessageBOOSTER™ cDNA Synthesis from Cell Lysates Kit enables the user to perform sensitive qRT-PCR studies from very small populations of cells, even from as little as one cell

BioSearch Tech (Lucigen/Epicentre)

Catalogue No.DescriptionPack SizePriceQty
MBCL90310MessageBooster™ cDNA Synthesis from Cell Lysates Kit10 Rxns £209.00£209.00Offer until : 31-Dec-2020Contact Cambio for special pricing on all orders over £2000 View Offer Quantity Add to Order

MessageBooster™ cDNA Synthesis from Cell Lysates Kit

The MessageBOOSTER™ cDNA Synthesis from Cell Lysates Kit enables the user to perform sensitive qRT-PCR studies from very small populations of cells, even from as little as one cell

BioSearch Tech (Lucigen/Epicentre)

 

Produce amplified amounts of cDNA from precious, limiting cell samples without bias

http://www.lucigen.com/images/Epicentre/i_messagebooster_lysates_procedure.gif?sfvrsn=0.6795364263563887

  • Perform RNA amplification directly from cultured cell lysates followed by cDNA synthesis
  • Amplify with this high-fidelity, linear RNA amplification process that preserves the relative transcript abundance of the sample.
  • Get more data out of precious samples – use less RNA for more RT-qPCR reactions
  • Readily and reproducibly detect even low-abundance transcripts from as little as one cell.
  • No need to purify RNA: Perform RNA amplification and cDNA synthesis reactions directly from cell lysates.
  • Collect samples less often and archive cDNA for future use, saving time and effort.

 

Applications

  • Amplification of mRNA directly from a cell lysate, without the need for purifying RNA, and conversion of the amplified RNA to cDNA.
  • Significantly increasing the number and sensitivity of qRT-PCRs from very small numbers of cells.
  • Generation of large amounts of cDNA from the mRNA of very small samples for archival purposes.

Figure 1 (click to enlarge). An overview of the procedure for the MessageBOOSTER™ cDNA Synthesis from Cell Lysates Kit. A kit reaction amplifies the poly(A) RNA (mRNA) directly from a crude cell lysate without the need for RNA purification. The amplified RNA is then reverse-transcribed to cDNA that can be diluted up to 1,000-fold for qPCR.

 

Figure 2. A MessageBOOSTER™ Kit reaction produces sufficient cDNA from a single-cell lysate for thousands of sensitive qPCRs. qPCR was performed using undiluted (red), 1:10 diluted (green), 1:100 diluted (blue), and 1:1,000 diluted (purple) cDNA from a lysate of a single NRK cell. The low-abundance PBGD transcript was readily detected.

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

MessageBooster™ cDNA Synthesis from Cell Lysates Kit

The MessageBOOSTER™ cDNA Synthesis from Cell Lysates Kit enables the user to perform sensitive qRT-PCR studies from very small populations of cells, even from as little as one cell

BioSearch Tech (Lucigen/Epicentre)

Protocols for: MessageBOOSTER™ cDNA Synthesis from Cell Lysates Kit

Due to the constant updating of the protocols by the manufacturer we have provided a direct link to Lucigen’s product page, where the latest protocol is available.

Please note this will open a new page or window on your computer.

 MessageBOOSTERTrade; cDNA Synthesis from Cell Lysates Kit Protocol

(catalogue number MBCL90310)

Please note: all protocols off site are the responsibility of the products supplier

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

MessageBooster™ cDNA Synthesis from Cell Lysates Kit

The MessageBOOSTER™ cDNA Synthesis from Cell Lysates Kit enables the user to perform sensitive qRT-PCR studies from very small populations of cells, even from as little as one cell

BioSearch Tech (Lucigen/Epicentre)

Applications

  • Amplification of mRNA directly from a cell lysate, without the need for purifying RNA, and conversion of the amplified RNA to cDNA.
  • Significantly increasing the number and sensitivity of qRT-PCRs from very small numbers of cells.
  • Generation of large amounts of cDNA from the mRNA of very small samples for archival purposes.

Benefits

  • Obtain up to thousands of qRT-PCRs from as little as one cell.
  • Readily and reproducibly detect even low-abundance transcripts from as little as one cell.
  • No need to purify RNA: Perform RNA amplification and cDNA synthesis reactions directly from cell lysates.
  • High-fidelity, linear RNA amplification process preserves the relative transcript abundance of the sample.
  • Collect samples less often and archive cDNA for future use, saving time and effort.

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200