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Oligo Synthesis

Oligo Synthesis : CEPs

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1-Me-dA-CE Phosphoramidite

1-Me-dA-CE Phosphoramidite

Glen Research

Catalogue No.DescriptionPack SizePriceQty
10-1501-021-Me-dA-CE Phosphoramidite 0.25g £600.00 Quantity Add to Order
10-1501-901-Me-dA-CE Phosphoramidite 100µmoles £200.00 Quantity Add to Order
10-1501-951-Me-dA-CE Phosphoramidite 50µmoles £100.00 Quantity Add to Order

1-Me-dA-CE Phosphoramidite

1-Me-dA-CE Phosphoramidite

Glen Research

1-Me-dA-CE Phosphoramidite

Structure

Catalog Number: 10-1501-xx

Description: 1-Me-dA-CE Phosphoramidite

5'-Monomethoxytrityl-N6-chloroacetyl-1-methyl-2'-deoxyAdenosine,
3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
Formula: C42H49ClN7O6P M.W.: 814.31 F.W.: 328.24

Diluent: Anhydrous Acetonitrile
Coupling: No changes needed from standard method recommended by synthesizer manufacturer. Monomers that allow for UltraMILD deprotection are recommended. (dA:10-1601-xx, dC: 10-1015-xx, dG: 10-1621-xx, dT: 10-1030-xx ). To avoid any exchange of the iPr-Pac group on the dG with acetyl, use the UltraMild Cap Mix A (40-4210-xx/ 40-4212-xx). 0.02M Iodine for oxidation is also recommended.
Deprotection: 2 M Ammonia in methanol, 24 hours at Room Temperature using UltraMILD amidites or 60 hours at Room Temperature if using standard amidites.
Storage: Refrigerated storage, maximum of 2-8°C, dry
Stability in Solution: Similar to dA,C,G,T-CE Phosphoramidites

MUTAGENESIS

Cellular polynucleotides are alkylated by endogenous components, such as S-adenosylmethionine, or after reacting with two general classes of environmental and laboratory chemicals. SN1 chemical agents include alkylnitrosourea and N-alkyl-N-nitro-N-nitrosoguanidine that react with the N7 position of guanine, N3 of adenine, O6 of guanine, O2 or O4 of pyrimidines, and the non-phosphodiester oxygen atoms of the phosphate backbone. In contrast, SN2 chemical agents such as methyl methanesulfonate and dimethyl sulfate react primarily with the N1 position of adenine (1-Methyl-2’-deoxyadenosine) and N3 of cytosine. To avoid chain branching during synthesis when using DCI as activator, N6-Me-dA is offered with acetyl protection.

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1-Me-dA-CE Phosphoramidite

1-Me-dA-CE Phosphoramidite

Glen Research

MSDS

Glen Report

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

1-Me-dA-CE Phosphoramidite

1-Me-dA-CE Phosphoramidite

Glen Research

DILUTION/COUPLING DATA

The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures.

ABI 392/394
Cat.No. Pack
Size
Grams/
Pack
0.1M Dil.
(mL)
LV40 LV200 40nm 0.2µm 1µm 10µm
Approximate Number of Additions
10-1501-90 100µmoles .081grams 1 20 12 7.5 5.45 4 1
10-1501-02 0.25grams .25grams 3.07 89 53.4 33.38 24.27 17.8 4.45
10-1501-95 50µmoles .041grams .5 3.33 2 1.25 .91 .67 .17
Expedite
Cat.No. Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity 50nm 0.2µm 1µm 15µm
Approximate Number of Additions
10-1501-90 100µmoles .081grams 1.5 .07 23.6 14.75 10.73 1.48
10-1501-02 0.25grams .25grams 4.58 .07 85.2 53.25 38.73 5.33
10-1501-95 50µmoles .041grams .75 .07 8.6 5.38 3.91 .54
Beckman
Cat.No. Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity 30nm 200nm 1000nm

Approximate Number of Additions
10-1501-90 100µmoles .081grams 1.5 .07 25.2 15.75 11.45

10-1501-02 0.25grams .25grams 4.58 .07 86.8 54.25 39.45

10-1501-95 50µmoles .041grams .75 .07 10.2 6.38 4.64

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