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Oligo Synthesis

Oligo Synthesis : Supports,CPGs

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3'-(6-Fluorescein) CPG

3'-(6-Fluorescein) CPG

Glen Research

Catalogue No.DescriptionPack SizePriceQty
20-2964-013'-(6-Fluorescein) CPG 0.1g £96.00£88.32Offer until : 31-Dec-2019Offer Code : GLEN88% off all Glen Research products View Offer Quantity Add to Order
20-2964-103'-(6-Fluorescein) CPG 1.0g £796.00£732.32Offer until : 31-Dec-2019Offer Code : GLEN88% off all Glen Research products View Offer Quantity Add to Order
20-2964-133'-(6-Fluorescein) CPG 1x10µm £240.00£220.80Offer until : 31-Dec-2019Offer Code : GLEN88% off all Glen Research products View Offer Quantity Add to Order
20-2964-143'-(6-Fluorescein) CPG 1x15µm £360.00£331.20Offer until : 31-Dec-2019Offer Code : GLEN88% off all Glen Research products View Offer Quantity Add to Order
20-2964-413'-(6-Fluorescein) CPG 4x1.0µm £160.00£147.20Offer until : 31-Dec-2019Offer Code : GLEN88% off all Glen Research products View Offer Quantity Add to Order
20-2964-423'-(6-Fluorescein) CPG 4x0.2µm £96.00£88.32Offer until : 31-Dec-2019Offer Code : GLEN88% off all Glen Research products View Offer Quantity Add to Order

3'-(6-Fluorescein) CPG

3'-(6-Fluorescein) CPG

Glen Research

3'-(6-Fluorescein) CPG

Structure

Catalog Number: 20-2964-xx

Description: 3'-(6-Fluorescein) CPG

1-Dimethoxytrityloxy-2-(6-carboxy-(di-O-pivaloyl-fluorescein)-4-aminobutyl)-
propyl-3-O-succinoyl-long chain alkylamino-CPG


F.W.: 566.48

Diluent: Not Applicable
Coupling: This support should be used in a manner identical to normal protected nucleoside support since it contains the DMT group.
Deprotection: Cleavage of the oligonucleotide from this support requires 2 hours at room temperature with ammonium hydroxide. Complete the deprotection using the protocol required by the nucleobases.
Storage: Freezer storage, -10 to -30°C, dry
Stability in Solution: Not Applicable

FLUORESCEIN LABELLING

5’-Fluorescein phosphoramidite contains no 4,4’-dimethoxytrityl (DMT) group and can be added only once at the 5’-terminus, thereby terminating synthesis. This product is prepared using the 6-carboxyfluorescein derivative. The tetrachloro-, hexachloro-and dichloro-dimethoxy-fluorescein phosphoramidites are designed to take advantage of the multicolor detection capability of modern DNA sequencers and genetic analyzers. Fluorescein phosphoramidite is designed to produce the same fluorescein-type structure as had been previously prepared using fluorescein isothiocyanate (FITC). Our fluorescein phosphoramidite also contains a DMT group to allow quantification of coupling. The analogous structure, 6-Fluorescein Phosphoramidite, prepared using 6-FAM, is also available, along with 6-Fluorescein Serinol Phosphoramidite. Fluorescein-dT can be inserted into the desired sequence as a replacement for a dT residue.

We offer five fluorescein supports. Fluorescein CPG has traditionally been used to add the fluorescein label at the 3’-terminus. The analogous structure, 3’-(6-Fluorescein) CPG, prepared using 6-FAM, is now also available, along with 6-Fluorescein Serinol CPG. We also offer 3’-(6-FAM) CPG and Fluorescein-dT CPG, both derivatives of 6-carboxyfluorescein (6-FAM). Both are single isomers and use an amide linkage which is stable during cleavage and deprotection and does not allow isomer formation. 3’-(6-FAM) CPG allows effective blockage of the 3’-terminus from polymerase extension as well as exonuclease digestion. Fluorescein-dT CPG allows both of these enzymatic activities to proceed. Normal cleavage and deprotection with ammonium hydroxide readily generates the fluorescein labelled oligos.

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

3'-(6-Fluorescein) CPG

3'-(6-Fluorescein) CPG

Glen Research

MSDS

Glen Report 13.1

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

3'-(6-Fluorescein) CPG

3'-(6-Fluorescein) CPG

Glen Research

QUESTION: Does AMA or methylamine cause any degradation to fluorescein or fluorescein-type dyes such as FAM or FITC?

RESPONSE:Response: While AMA (Ammonium hydroxide/40% Methylamine 1:1 v/v) is considered compatible with fluorescein, the use of methylamine when deprotecting a Fluorescein-labeled oligo does lead to a small amount of degradation, which is characterized by a the appearance of a late-eluting peak by RP HPLC that shows no visible fluorescein absorbance. With standard deprotection conditions (AMA 10 minutes at 65 C) the amount of degradation is approximately 5%. The impurity is not detected with AMA at RT for 2 hours.

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200