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Oligo Synthesis

Oligo Synthesis : Supports,CPGs

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3'-BHQ-3 CPG

3'-BHQ-3 CPG

Glen Research

Catalogue No.DescriptionPack SizePriceQty
20-5933-013'-BHQ-3 CPG 0.1g £152.00£139.84Offer until : 31-Dec-2019Offer Code : GLEN88% off all Glen Research products View Offer Quantity Add to Order
20-5933-103'-BHQ-3 CPG 1.0g £1,200.00£1,104.00Offer until : 31-Dec-2019Offer Code : GLEN88% off all Glen Research products View Offer Quantity Add to Order
20-5933-133'-BHQ-3 CPG 1x10µm £460.00£423.20Offer until : 31-Dec-2019Offer Code : GLEN88% off all Glen Research products View Offer Quantity Add to Order
20-5933-143'-BHQ-3 CPG 1x15µm £660.00£607.20Offer until : 31-Dec-2019Offer Code : GLEN88% off all Glen Research products View Offer Quantity Add to Order
20-5933-413'-BHQ-3 CPG 4x1.0µm £240.00£220.80Offer until : 31-Dec-2019Offer Code : GLEN88% off all Glen Research products View Offer Quantity Add to Order
20-5933-423'-BHQ-3 CPG 4x0.2µm £64.00£58.88Offer until : 31-Dec-2019Offer Code : GLEN88% off all Glen Research products View Offer Quantity Add to Order

3'-BHQ-3 CPG

3'-BHQ-3 CPG

Glen Research

3'-BHQ-3 CPG

Structure

Catalog Number: 20-5933-xx

Description: 3'-BHQ-3 CPG

3-Diethylamino-5-phenylphenazium-7-diazobenzene-4''-(N-ethyl-
2-O-(4,4'-dimethoxytrityl))-N-ethyl-2-O-glycolate-CPG


F.W.: 597.63

Diluent: Anhydrous Acetonitrile
Coupling: UltraMILD monomers should be used. (dA: 10-1601-xx, dC: 10-1015-xx, dG: 10-1621-xx, dT: 10-1030-xx). To avoid any exchange of the iPr-Pac group on the dG with acetyl, use the UltraMild Cap Mix A (40-4210-xx/40-4212-xx).
Deprotection: Deprotect with 30% Ammonium Hydroxide for 2 hours at Room Temperature. Note: BHQ-3 is unstable to potassium carbonate in methanol.
Storage: Freezer storage, -10 to -30°C, dry
Stability in Solution: 2-3 days
Please Note: 'Black Hole Quencher', 'BHQ-0', 'BHQ-1', 'BHQ-2' and 'BHQ-3' are trademarks of Biosearch Technologies, Inc., Novato, CA. The BHQ dye technology is the subject of pending patents and is licensed and sold under agreement with Biosearch Technologies, Inc.. Products incorporating the BHQ dye moiety are sold exclusively for R&D use by the end-user. They may not be used for clinical or diagnostic purposes and they may not be re-sold, distributed or re-packaged.

Black Hole Quencher Dyes

With the growing popularity of red and near-infrared dyes, we are offering the Black Hole QuencherTM dyes (BHQs), whose physical properties are detailed in Table 1. BHQ dyes are robust dark quenchers that very nicely complement our existing product line. They are compatible with ammonium hydroxide deprotection, exhibit excellent coupling efficiencies, have large extinction coefficients and are completely non-fluorescent. Their absorbances are well-tuned to quench a variety of popular fluorophores – even those far into the red, such as Cy3 and Cy5. The dark quencher most typically used in a Molecular Beacon is Dabcyl. Because the quenching does not involve FRET, there is little, if any, dependence upon donor-acceptor spectral overlap. In a comprehensive paper by Marras, Kramer and Tyagi,1 the ability of BHQ-1 and BHQ-2 to quench 22 different fluorophores was evaluated. For shorter wavelength fluorophores such as fluorescein, the quenching efficiency was roughly the same as Dabcyl (91% – 93%). However, for dyes emitting in the far red, such as Cy5, the BHQ dyes were far superior – quenching the Cy5 with 96% efficiency, compared to 84% with Dabcyl. This may reflect the BHQ’s ability to form stable, non-fluorescent complexes which can be a plus even in FRET probes. Indeed, recent work suggests that these non-fluorescent complexes will form even in the absence of a hairpin stem structure used by Molecular Beacons.2

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3'-BHQ-3 CPG

3'-BHQ-3 CPG

Glen Research

MSDS

Glen Report 17.1

Glen Report 21.2

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

3'-BHQ-3 CPG

3'-BHQ-3 CPG

Glen Research

QUESTION: What are the physical properties of the various black hole quenchers?

RESPONSE:

Quencher lmax (nm) E260 (L/mol.cm) Emax (L/mol.cm)
BHQ-0 493 7,700 34,000
BHQ-1 534 8,000 34,000
BHQ-2 579 8,000 38,000
BHQ-3 672 13,000 42,700

REFERENCE(S):
Glen Report 17.1

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200