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Oligo Synthesis

Oligo Synthesis : Supports, CPGs

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3'-Dabcyl CPG

3'-Dabcyl CPG

Glen Research

Catalogue No.DescriptionPack SizePriceQty
20-5912-013'-Dabcyl CPG 0.1g £109.00 Quantity Add to Order
20-5912-103'-Dabcyl CPG 1.0g £886.00 Quantity Add to Order
20-5912-133'-Dabcyl CPG 1x10µm £318.00 Quantity Add to Order
20-5912-143'-Dabcyl CPG 1x15µm £455.00 Quantity Add to Order
20-5912-413'-Dabcyl CPG 4x1.0µm £182.00 Quantity Add to Order
20-5912-423'-Dabcyl CPG 4x0.2µm £109.00 Quantity Add to Order

Description

3'-Dabcyl CPG

Structure

Catalog Number: 20-5912-xx

Description: 3'-Dabcyl CPG

1-Dimethoxytrityloxy-3-[O-(N-4'-carboxy-4-(dimethylamino)-
azobenzene)-3-aminopropyl)]-propyl-2-O-succinoyl-long chain alkylamino-CPG


F.W.: 462.44

Diluent: Not Applicable
Coupling: This support should be used in a manner identical to normal protected nucleoside support since it contains the DMT group.
Deprotection: Cleavage of the oligonucleotide from this support requires 2 hours at room temperature with ammonium hydroxide. Complete the deprotection using the protocol required by the nucleobases.
Storage: Freezer storage, -10 to -30°C, dry. Light sensitive material.
Stability in Solution: Not Applicable

Dabcyl LABELLING

A molecular beacon probe1 has its natural fluorescence quenched in solution unless it is hybridized to the target sequence. Consequently, the design of a molecular beacon requires a fluorophore to be in one part of the sequence and the quencher molecule to be in another, with both molecules being separated from the oligonucleotide by a hydrocarbon spacer. The Dabcyl group has been found to be a universal quencher. 3’-Dabsyl CPG and 3’-Dabcyl CPG are used to prepare probes with the quencher blocking the 3’-terminus. 5’-Dabcyl Phosphoramidite locates the quencher at the 5’-terminus and Dabcyl-dT places it within the sequence, leaving the 3’-terminus available for polymerase extension.

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Protocols

MSDS

Glen Report 12.1

Glen Report 12.1

Glen Report 21.2

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Applications & Benefits

EXTINCTION DATA

Item Nucleoside λMax-1 Emax-1 λMax-2 Emax-2 E260


(nm) (ml/µmole) nm (ml/µmole) (ml/µmole)
20-5912
478 32

13.9

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Related products

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